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中文摘要：
目的研究骨桥蛋白（osteopontin，OPN）下调后对胶质瘤细胞侵袭和凋亡的影响。方法在体外以慢病毒介导的OPN小干扰RNA（smallinterference，siRNA）感染胶质瘤细胞系U251细胞，实时PCR和Western印迹检测OPN表达。通过Transwell实验、流式细胞仪检测，观察OPN表达下调后对胶质瘤细胞侵袭和细胞凋亡的影响。结果慢病毒介导的OPNsiRNA感染能显著降低U251细胞0PNmRNA水平及蛋白表达，有效抑制U251细胞侵袭能力，并诱导其凋亡，0PNsiRNA感染组中Bcl-2、尿激酶型纤溶酶原激活剂（uPA）、MMP-2和MMP-9明显减少，Bax显著增多。结论慢病毒介导的0PNsiRNA可显著抑制U251细胞的侵袭，并促进其凋亡。
英文摘要：
Objective To investigate the effect of osteopontin silencing on the invasion and apoptosis of U251 cells. Methods The invasion, apoptosis and levels of uPA, MMP-2 and MMP-9 were determined by invasion assay, flow cytometry, Western blot and real-time fluorescence quantitative PCR respectively. Results Osteopontin small interference （siRNA） inhibited osteopontin expression and cell invasion, promoted apoptosis in U251 cells. In addition, the expression of Bcl-2, uPA, MMP-2 and MMP-9 was decreased, while Bax level was elevated. Conclusion Osteopontin siRNA can inhibit U251 cells invasion via the down-regulation of uPA, MMP-2 and MMP-9 levels, and promote apoptosis through induction of Bax expression and inhibition of Bcl-2 level. It suggests that osteopontin plays an important role in human glioma progression.
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