中文摘要：

目的 探讨高脂高糖饮食对自发性高血压大鼠（SHR）水通道蛋白1（AQP-1）mRNA与NO表达的影响,及其在加重尿钠排泄中的作用.方法 6周龄雄性SHR24只随机分为高脂高糖组12只和普通饲料组12只,第8、12周末观察高脂高糖饮食对其NO、尿钠排泄等的影响,并计算肾质量指数.12周末RT-PCR检测肾脏AQP-1mRNA表达.结果 与普通饲料组比较,高脂高糖组第8、12周末血压、血浆NO、肾脏尿钠排泄量明显降低（P＜0.05）,12周末肾组织AQP-1 mRNA表达明显降低[（0.54±0.07） vs （1.28±0.39）,P＜0.01],第12周末肾质量指数明显升高[（3.40±2.00）mg/g vs （2.30±1.00）mg/g,P＜0.05].结论 长期高脂高糖饮食可加重SHR肾脏损害,其机制可能与内源性NO合成降低、肾脏AQP-1 mRNA表达降低相关.

英文摘要：

Objective To study the effect of high fat and glucose diet on expression of aquaporin-1 （AQP-1） mRNA and excretion of urine natrium in SHR.Methods Twenty-four male SHR aged 6 weeks were randomly divided into high fat and glucose diet group （n=12） and normal diet group （n=12）.Effect of high fat and glucose diet on NO synthase and excretion of urine natrium was detected at weeks 8 and 12.The animals were killed at the end of week 12 to harvest the total RNA.Expression of AQP-1 mRNA was detected by RT-PCR.Results The serum NO synthase level,urine natrium excretion volume and AQP-1 mRNA expression level were significantly lower in high fat and glucose diet group than in normal diet group at weeks 8 and 12.Conclusion Long-term high fat and glucose diet aggravates kidney damage in SHR by downregulating their endogenous NO synthesis and AQP-1 mRNA expression in kidneys.