中文摘要：

目的 探讨低氧诱导因子1α（HIF-1α）在组织缺血时血管生成及内皮祖细胞（EPCs）动员中的作用。方法 SD雌性大鼠36只，随机分为下肢缺血加HIF-1α反义寡核苷酸组（HIF-1α反义寡核苷酸组）、下肢缺血加HIF-1α错义寡核苷酸组（HIF-1α错义寡核苷酸组）和单纯下肢缺血组，每组12只，于股动脉结扎建立下肢缺血模型，缺血局部微注射脂质体转染的全硫代修饰HIF-1α寡核苷酸。测血浆血管内皮生长因子（VEGF）；acLDL-DiI和FITC-UEA-Ⅰ双染色鉴定EPCs后计数；下肢缺血区肌肉组织测HIF-1α mRNA和VEGF mRNA的表达和术后28天缺血区肌肉组织毛细血管密度。结果HIF-1α反义寡核苷酸组的HIF-1α mRNA和VEGF mRNA表达均较单纯下肢缺血组和HIF-1α错义寡核苷酸组明显减少；HIF-1α反义寡核苷酸组的循环EPCs、血浆VEGF及缺血区肌肉组织毛细血管密度均较单纯下肢缺血组和HIF-1α错义寡核苷酸组明显减少。结论 全硫代修饰HIF-1α反义寡核苷酸可抑制大鼠缺血肢体血管生成和因缺血引起的EPCs的动员，其机制可能是通过VEGF起作用，HIF-1α是组织缺血时EPCs一个动员因子。

英文摘要：

Objective To study the effect of hypoxia-inducible factor- 1 α（ HIF- 1 α） on angiogenesis and endothelial progenitor cell（EPCs） mobilization during tissue ischemia. Methods Thirty-six rats were randomly divided into three groups：hind limb ischemia group, hind limb ischemia plus HIF-1α antisense oligonucleotide group and hind limb ischemia plus HIF-1α missense oligonucleotide group. Hind limb ischemia model was established by ligation of femoral artery. On the 7th day after operation and treatment with HIF-1α oligonucleotide, HIF-1α mRNA and VEGF mRNA were measured by RT-PCR. Circulating EPCs and plasma VEGF were also measured. Twenty-eight days after the operation, capillary density was measured in ischemic muscles. Results Compared with the hind limb ischemia group and hind limb ischemia plus HIF-1α missense oligonucleotide group, the expression of HIF-1α mRNA and VEGF mRNA, circulating EPCs and capillary density were decreased in hind limb ischemia plus HIF-1α antisense oligonucleotide group. Conclusions HIF-1α antisense oligonucleotide can inhibit angiogenesis and circulating endothelial progenitor cell mobilization in rat hind limb ischemia through regulating role of the VEGF. HIF-1α is a mobilization factor of EPCs during tissue ischemia.