中文摘要：

目的探讨高架十字迷宫实验（elevated plus maze，EPM）作为昆明小鼠状态焦虑动物模型的重测信度。方法昆明小鼠（4周龄，♂／♀）进行EPM实验，实验间隔1周进行2次，采用摄像系统记录5min行为变化；纳入实验参数有：开臂区进入时间百分率（Otime％），闭臂区进入时间百分率（Ctime％），开臂区进入时间（Otime），闭臂区进入时间（Ctime），开臂区进入次数百分率（Oentries％），开臂区进入次数（Oentries），闭臂区进入次数（Centries）及两臂区进入总次数（Entries）；通过组内相关系数（intraclass correlation coefficient，ICC）和kappa一致性系数（kappa agreement index，κ）对EPM进行重测信度检验。结果ICC结果显示，与初测相比，Otime％（♂ ICC=0．506，P〈0．05；♀ ICC=0．456，P〈0．05；♂＋♀ICC=0．492，P〈0．01）、Oentries（♂ICC=0．673，P〈0．01；♀ICC=0．648，P〈0．01；♂＋♀ICC=0．665，P〈0．01）、Centries（♂ICC=0．704，P〈0．01；♀ICC=0．661，P〈0．01；♂＋♀ICC=0．686，P〈0．01）、Entries（♂ICC=0．701，P〈0．01；♀ICC=0．661，P〈0．01；♂＋♀ICC=0．685，P〈0．01）重测信度相对较好，且差异有统计学意义。κ结果显示，与初测相比，仅Entries（♂κ=0．253，P〈0．05；♀κ=0．339，P〈0．05；♂＋♀κ=0．298，P〈0．01）重测信度相对较差，且差异亦有统计学意义。结论Otime％、Entries作为EPM评价状态焦虑动物模型的稳定参数，具有尚可接受的重测信度；♂／♀及重复测量对EPM实验参数Otime％、Entries均有不同程度影响，但对实验参数Otime％影响相对较大。因此，EPM作为昆明小鼠状态焦虑动物模型进行药效学评价时，实验参数推荐Otime％、Entries，实验动物推荐♂，并应注意♂／♀及重复测量效应对实验参数Otime％影响。

英文摘要：

Objective To explore the test-retest reliability of elevated plus maze （EPM） which is taken as an animal model of state anxiety in Kunming mice. Methods The behavior of adult, male and female, Kunming mice in EPM was recorded twice with one-week inter-trial interval for lasting five minutes.The following parameters were evaluated ： percentage of time on EPM open arms （ Otime% ） , percentage of time on EPM closed arms （Ctime%） , time on EPM open arms （Otime） , time on EPM closed arms （Ctime） , percentage of entries into EPM open arms （Oentries%）, entries into EPM open arms （Oentries） ,entries into EPM closed arms （Centries）, total entries into EPM open and closed arms （Entries）. Subsequently, the intraclass correlation coefficient （ICC） and the kappa agreement index （κ） were calculated for these parameters. Results ICC for Otime% （ ♂ ICC=0.506, P〈0.05; ♀ ICC=0.456, P〈0.05; ♂ ＋ ♀ ICC=0.492, P 〈0.01） ,Oentries （ ♂ ICC=0.673, P〈0.01; ♀ ICC=0.648, P〈0.01; ♂ ＋ ♀ ICC=0.665, P〈0.01） ,Centries （ ♂ICC=0.704, P〈0.01; ♀ ICC=0.661, P〈0.01; ♂ ＋ ♀ ICC=0.686, P〈0.01） ,Entries （ ♂ICC= 0.701, P〈0.01 ; ♀ ICC = 0.661, P〈0.01;♂ ＋ ♀ICC = 0.685, P〈0.01） showed a fair test-retest reliability, respectively. Only K for Entries （ ♂ κ=0.253, P〈0.05; ♀ κ=0.339, P〈0.05; ♂ ＋ ♀κ=0.298, P〈0.01） displayed a significant but poor reliability between the test-retest trials. Conclusion The Otime% Entries parameters as an acceptable index for Kunming mice in EPM can be used for exploratory and locomotion activity. Sex and repeated measurement have different effects on the Otime% and Entries parameters, especially the more on the former. Therefore, Otime% and Entries can be considered when EPM as an animal model of state anxiety in Kunming mice; meanwhile, more attention should be paid to both the sex （male animals recommended to EPM ,especially） and repeated measurement effects.