中文摘要：

通过根据糖海带（Laminaria saccharina）叶绿素a／c结合蛋白基因lhcf6的cDNA序列设计的引物和PCR方法获得了海带（L．japonica）配子体lhcf6基因的cDNA全长序列（GenBank登录号：DQ250739）。该序列的开放阅读框（ORF）与糖海带lhcf6编码序列的相似性高达99％，而两非翻译区（UTR）序列的相似性只有94％。经推测，海带配子体lhcf6基因序列的ORF编码一个含218个氨基酸的前体蛋白LHCF6，其氨基端的40个氨基酸组成跨质体内质网和叶绿体膜的信号肽，跨膜酶切后变为含178个氨基酸的成熟蛋白，它的分子量为19．3kD，等电点为4．88。预测的成熟蛋白LHCF6高级结构存在2个保守性的β折叠和3个跨类囊体膜的α螺旋区。根据海带配子体LHCF6成熟蛋白以及GenBank中12个同源蛋白质的氨基酸序列所构建的Neighbor-joining分子进化树，显示藻类在光捕获蛋白氨基酸序列水平上存在着蓝藻与红藻、杂色藻类、裸藻与绿藻等三个方向的演化途径，其中裸藻和绿藻与高等植物的亲缘关系更近。

英文摘要：

The complete coding sequence （cds） of lhcf6 gene from Laminaria japonica gametophytes was obtained by using a PCR based method and primers designed according to the lhcf6 cds from L. saccharina. The sequence comparison of the open reading frame from this gene with its homolog in L. saccharina revealed the sequence similarity of 99 %, but that of their untranslated regions was only 94%. The precursor protein was deduced to be composed of 218 amino acids and the first 40 amino acids from the terminal of NH2 constituted plastid endoplasmic reticulum and chloroplast transmembrane signal peptides. The mature protein from enzymatieal digestion after mambrane transferring contained 178 amino acids, a putative molecular weight of 19.3 kD and PI of 4.88. The deduced amino acid sequence was completely identical with LHCF6 from L. saccharina, containing three membrane-spanning a-helices and two β-strands. The protein sequence of LHCF6 from L. japonica and twelve other proteins containing a chloroa_ b-bind domain from ten different algal species and one higher plant species, Arabidopsis thaliana, were selected for algae lineage divergence analysis. The Neighborjoining phylogenetic tree constructed by MEGA2.0 revealed three significantly different lineages from algae： blue-green/ red algae, chromophytes, and green algae/Eug/ena. Green algae and Eug/ena were clustered with the higher plant.