中文摘要：

为调查表皮葡萄球菌和腐生葡萄球菌在东北地区的流行病学情况和耐药性,本研究对来自东北地区3个大型奶牛场采集的330份奶样进行葡萄球菌的分离、鉴定及其耐药表型的检测,并采用脉冲场凝胶电泳（PFGE）进行分离株的亲缘性分析,对表皮葡萄球菌进行多位点序列分型（MLST）,同时应用PCR扩增分离株中携带的相关耐药基因。研究结果表明,在330份奶样中共分离到表皮葡萄球菌32株（9.7%）,腐生葡萄球菌34株（10.3%）;PFGE分析共获得9种不同谱型的表皮葡萄球菌和11种不同谱型的腐生葡萄球菌。药敏试验结果显示,两种菌对青霉素（70%）、苯唑西林（60%）和林克霉素（55%）的耐药率较高,主要耐药基因为lnu（B）（40%）、erm（B）（30%）和mec A（25%）。本研究结果揭示了东北地区奶牛乳房炎病原菌表皮葡萄球菌和腐生葡萄球菌的耐药谱和流行情况,为临床合理用药及奶牛乳房炎的防控提供了实验依据。

英文摘要：

Staphylococcus epidermidis and S.saprophyticus are the major pathogens for the disease of the bovine mastitis. In order to investigate antimicrobial resistance and the prevalence of S.epidermidis and S.saprophyticus in the Northeast China, a total of bovine mastitis affected 330 milk samples were collected form 3 different dairy farms in part of Northeast China, and subjected to Staphylococcus isolations, the genetic relationship（pattern） of the isolates by the pulsed-filed gel electrophoresis（PFGE） and multilocus sequence typing（MLST） identifications, and antimicrobial susceptibility tests and resistance genes detections. The results shown that 66 Staphylococcus were isolated, including 32（9.7%） S.epidermidis and 34（10.3%） S.saprophyticus. PFGE analysis revealed that 32 S.epidermidis and 34 S.saprophyticus were divided into 9 and 11 different PFGE patterns, respectively.All isolates displayed high resistant rate to penicillin（70%）, oxacillin（60%） and lincomycin（55%）, and the rates of main resistance genes were 40% for lnu（B）, 30% for erm（B） and 25% for mec A, respectively. In conclusion, this study revealed the antimicrobial resistance profiles and molecular epidemiology of S.epidermidis and S.saprophyticus in part of Northeast China.