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SNP和cPTIO对NaCl胁迫下拟南芥的生理影响
  • 期刊名称:广西植物 2010, 30(5):666-671
  • 时间:0
  • 分类:Q945[生物学—植物学]
  • 作者机构:[1]昆明学院生命科学与技术系,昆明650214, [2]云南大学生命科学学院,昆明650091, [3]云南师范大学生命科学学院,昆明650092
  • 相关基金:国家自然科学基金(30860121); 中国农业大学植物生理学与生物化学国家重点实验室开放课题基金(PPB08010)
  • 相关项目:APAM2基因在拟南芥花粉发育中的功能研究
中文摘要:

以拟南芥为材料,研究了0.5mmol/L的外源一氧化氮供体硝普纳(SNP)和200mmol/L的一氧化氮专一清除剂(cPTIO)对200mmol/LNaCl胁迫下对拟南芥的生理影响。0.5mmol/LSNP和200mmol/LcPTIO预处理2h后,加入200mmol/LNaCl。不同处理的拟南芥加入200mmol/LNaCl后,每隔12h取样一次,观察叶片生长情况、测定过氧化物酶(POD)、超氧化物歧化酶(SOD)、丙二醛(MDA)和超氧阴离子(O2^·-)的变化。结果表明0.5mmol/L的SNP能缓解200mmol/LNaCl胁迫伤害,促进盐胁迫下幼苗的生长,显著提高抗氧化酶系统中POD和SOD活性,显著降低MDA和O2^·-的含量,从而提高植株的抗盐性,而NO专一清除剂cPTIO能逆转SNP的上述效应。

英文摘要:

Three groups of 8-week-old Arabidopsis seedlings were cultured,they were respectively pretreated with water,0.5 mmol/L SNP and 200 mmol/L cPTIO for 2 h,after that,200 mmol/L NaCl was added. Fresh NaCl solution was applied every day and some physiological indices such as peroxidase(POD),superoxide dismutase(SOD),malondialdehyde(MDA)and superoxide anion(O2^·-)were determined 12 h later. The results showed that SNP could relieve NaCl stress injuries,and promote the growth of seedlings under salt stress,significantly improve the antioxidant enzyme system,POD and SOD activity,significantly reduce MDA and O2^·- content,thereby enhancing the salt tolerance of plants,while the NO specific scavenger cPTIO could reverse the above effects of SNP.

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