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中文摘要：
利用圆二色性光谱和荧光光谱对鸡蛋溶菌酶的热变性过程进行了系统的光谱学研究,得到了鸡蛋溶菌酶热变性过程的光谱学特征;通过酶联免疫吸附实验测定鸡蛋溶菌酶热变性过程的免疫原性变化;结合生物信息学方法,分析了热变性过程中鸡蛋溶菌酶蛋白结构改变与其免疫原性变化之间的关系,建立研究食品过敏原蛋白热变性的光谱学方法。
英文摘要：
Thermal denaturation for lysozyme protein of egg has been investigated by using circular dichroism spectra and fluorescence spectra systematically.The corresponding immunogenicity of lysozyme protein has been tested with ELISA experiments.In addition,bioinformatics methods have also been used to investigate the spectral characteristics,corresponding immunogenicity change and conformational changes of the protein during thermal denaturation.Results from this study should be useful to the establishment of a spectral method examining the extent of thermal denaturation of the protein.
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