中文摘要：

目的：探讨外源性甲胎蛋白（ AFP）对体外培养的肝癌细胞系的生物学功能及意义。方法该实验选用了HepG2（AFP阳性）和HLE（AFP阴性）两种常见的肝癌细胞系，再加入不同浓度的外源性AFP处理24 h后，用MTT检测细胞的增殖情况；在这两种细胞系中加入凋亡诱导剂ATRA处理的同时加入外源性AFP，检测AFP对于细胞的抗凋亡能力的影响。结果在HepG2和HLE细胞中，加入不同浓度的外源性AFP后， MTT结果显示AFP可以明显促进肝癌细胞的增殖；而加入凋亡诱导剂ATRA之后，FCM结果显示细胞凋亡明显增加，加入外源性AFP后，细胞凋亡的情况明显好转，外源性AFP可以增强肝癌细胞的抗凋亡能力。结论外源性AFP不仅可以促进肝癌细胞系的增殖，同时还可以增强肝癌细胞的抗凋亡能力。

英文摘要：

Objective To investigate the biological functions and significance of exogenous AFP in hepatoma cell lines.Methods Two types of normal hepatoma cell lines （ HepG2 and HLE） were chosen,added with different concentrations of exogenous AFP for 24 hours,MTT was used to detect cells proliferation;ATRA and AFP were employed to stimulate cells at the same time,FCM was used to detect cells apoptosis.Results For the HepG2 and HLE cells with exogenous AFP, MTT results showed that AFP could increase cell proliferation significantly.Comparing with control group,when cells were treated with ATRA alone,cells apoptosis were significantly increased,while after adding exogenous AFP,cell apoptosis were partially reversed.Conclusion Exogenous AFP can not only promote the proliferation of hepatoma cell lines,but also enhance the cells anti-apoptosis ability.