中文摘要：

目的探讨颈髓1-2后角星形胶质细胞对偏头痛大鼠模型痛觉超敏的影响。方法54只SD大鼠按随机数字表法分为9组：空白组、假手术组、新型致炎剂炎性汤（inflammatory soup，IS）3d组（IS3d组）、新型致炎剂7d组（IS7d组）、生理盐水组、氟代柠檬酸（FCA）预防组（iS-FCA预防组）、FCA预防对照组（IS-NS预防组）、FCA治疗组（IS-FCA治疗组）和FCA治疗对照组（IS-NS治疗组）。采用新型致炎剂刺激大鼠硬脑膜模型，应用Von-Frey纤维丝测定大鼠眶周皮肤的痛觉阈值，免疫荧光技术观察各组大鼠颈髓1-2后角神经元激活标志物C-fos和星形胶质细胞特异性蛋白胶质纤维酸性蛋白（GFAP）的表达。结果①Von-Frey结果：与对照组阈值相比，IS3d组、IS 7d组疼痛阈值下降明显（分别为3d组：6．4±0．3与5．3±0．3，F=40．15；7d组：6．3±0．2与3．0±0．3，F=382．5，均P〈0．01），IS-FCA预防组、iS．FCA治疗组疼痛阈值均在给予FCA第4天明显升高（预防组：5．5±5．I与5．1±0．2，F=16．00；治疗组：4．3±0．2与3．0±0．2，F=138．0，均P〈0．01）。②GFAP免疫荧光：Is3d、IS7d组高于对照组（吸光度值分别为：3d：24．5±4．4与14．8±2．5，F=32．54；7d：38．9±7．1与14．6±1．8，F=63．56，均P〈0．01）。③C-fos免疫荧光：IS3d组较对照组增高（20．4±2．3与8．4±1．1，F=129．0，P〈0．01），IS7d组与对照组差异无统计学意义。结论在慢性硬脑膜炎症所致的偏头痛痛觉超敏中，我们发现了星形胶质细胞的激活，且其特异性抑制剂FCA能有效预防并缓解痛觉超敏，提示星形胶质细胞可能在启动和维持痛觉超敏阶段均发挥着重要作用。

英文摘要：

Objective To explore the effects of astrocytes in cervical spinal cord posterior horn on allodynia in rat models of migraine. Methods Fifty-four male SD rats were randomly divided into 9 groups （ each group n = 6 ） ： blank group, sham surgery group, new inflammatory soup 3 d group （ IS 3 d group ）, new inflammatory soup 7 d group（ IS 7 d group）, saline group, fluoroeitrate （FCA）prevention group, FCA prevention control group, FCA treatment group, FCA treatment control group. Following the IS stimulation in the rat dual matter, Von-Frey hair was used to monitor the pain threshold of periorbital skin. Immunofluorescenee technique was used to observe the expression of specific marker of astrocyte, glial fibrilary acidic protein （ GFAP）, and neuron activation marker, C-fos. Results ①Von-Frey hair study showed the thresholds of IS 3 d group and IS 7 d group were significantly decreased compared with the control group（3 d：5.3 ±0.3 vs 6.4 ±0. 3,F =40. 15;7 d.3.0 ±0.3 vs 6.3 ±0.2,F =382. 5, both P 〈0.01）. The pain thresholds of FCA prevention group and FCA treatment group both began to significantly increase at the day 4 （ FCA prevention： 5.5 ± 5.1 vs 5. 1 ± 0. 2, F = 16. 00 ; FCA treatment ： 4. 3 ± 0. 2 vs 3.0 ± 0. 2, F = 138. 0, both P 〈 0. 01 ）. ②GFAP immunofluorescenee showed that the mean optical density （A） values of IS 3 d group and IS 7 d group were significantly increased compared with the control group（3 d：24. 5 ±4. 4 vs 14.8± 2.5, F = 32.54;7 d：38.9 ± 7.1 vs 14.6 ± 1.8, F = 63.56, both P 〈 0.01） .③C-fos immunofluorescence showed that mean A value of IS 3 d group was significantly increased compared with the control group （20.4 ±2. 3 vs 8.4 ± 1.1 ,F = 129.0 ,P 〈 0. 01 ）. The difference was not significant between the IS 7 d group and the control group. Conclusions Activated astrocytes contribute to the facial allodynia induced by chronic dural inflammation. Its inhibitor FCA could both prevent and treat the allodynia behaviour. All o