中文摘要：

目的：完善卷柏的质量标准。方法：依据《中国药典》2010年版附录相关方法,对卷柏药材进行显微鉴别,对灰分、酸不溶性灰分进行检查。采用HPLC检测selaginellin和穗花杉双黄酮的含量,选用Waters Cosmosil C18柱（250 mm×4.6 mm id,5μm）;检测波长：300、337 nm;流速：1 m L·min-1;柱温：30℃;流动相：乙腈-0.1%甲酸水梯度洗脱。结果：确定了卷柏药材的显微特征;酸不溶性灰分限度≤11.0%,总灰分限度≤15.0%;建立同时测定卷柏药材中穗花杉双黄酮和selaginellin的HPLC含量检测方法。结论：卷柏药材的显微鉴别,以及同时测定穗花杉双黄酮和selaginellin的HPLC含量测定等方法简便、准确、重现性好,可有效控制卷柏药材的质量。

英文摘要：

Objective： To improve quality standards of Herba Selaginellae. Methods： According to the methods recorded in the appendix of Chinese Pharmacopeia（2010 edition）, the microscopic identification ofHerba Selaginellae was conducted, and the tests of ash and acid-insoluble ash were carried out. The contents of bioactive constituents in selaginellin and amentoflavone were determined by HPLC on a Waters Cosmosil C18 column（250 mm× 4.6 mm id, 5 μm） and eluted with acetonitrile-0.1% formic acid as the mobile phase at a flow rate of 1 m L·min-1.The column temperature was 30 ℃ and the detection wavelength was 300 nm and 337 nm.Results： Microscopic characteristics were identified. The limit for acid insoluble ash was ≤ 11.0%, and the total ash limit was ≤ 15.0%. The HPLC method for simultaneous determination of amentoflavone andSelaginellin was established. Conclution： The developed method is accurate, sensitive and reproducible, which is effective for the quality control of Herba Selaginellae.