中文摘要：

目的探讨育龄期慢性前列腺炎患者精液中细菌、解脲脲支原体（Uu）和沙眼衣原体（CT）分布与耐药性，为临床诊断及治疗提供依据。方法2011年7月-2012年12月医院就诊的慢性前列腺炎患者185例，采集育龄期慢性前列腺炎患者的精液进行细菌、Uu培养和体外药敏试验，及CT-DNA采用荧光定量PCR方法检测，对结果采用SPSS13．0软件进行统计分析。结果185例育龄期慢性前列腺炎患者的精液中病原体总检出率为71．4％，检出细菌中革兰阳性菌占67．0％，以无乳链球菌最多，革兰阴性菌占33．0％，以大肠埃希菌最多，检出4株阴道加德纳菌；无乳链球菌对克林霉素、四环素、红霉素耐药率52．9％～88．0％，大肠埃希菌对氨苄西林、四环素、哌拉西林耐药率较高，Uu对环丙沙星耐药率为63．6％。结论细菌、Uu和CT感染是引起育龄期慢性前列腺炎的主要病原体，部分病原体来自女性生殖道菌群，细菌和Uu对常用抗菌药物已产生了一定的耐药性，应密切监控夫妇间交叉感染和药物耐药性，以提高临床治愈率。

英文摘要：

OBJECTIVE To investigate the distribution and drug resistance of pathogens, Ureaplasma urealyticum （Uu）and Chlarnydia trachomatis （CT） in the semen of chronic prostatitis patients in reproductive stage, so as to provide reference for clinical diagnosis and treatment. METHODS 185 chronic prostatitis patients were selected who were hospitalized during Jul. 2011-2012, their semen collected were to culture the bacteria and Uu extracted from the semens, and in vitro drug resistance test was takem; CT-DNA was detected by real-time fluorogenic quantitative PCR, and statistical analysis was made for results with SPSS 13.0 software. RESULTS Among 185 patients, the pathogen detected in their semen accounted for 71.4 %, of which gram positive bacteria accounted for 67.0%, and Streptococcus agalactiae was the majority; gram negative bacteria accounted for 33.0%, and most of them were Escherichia coli. 4 cases of Gardnerella vaginalis were also found. The drug sensitive test indicated that, the drug resistance rate of S. agalactiae against to clindamycin （CLDM）, tetracycline and erythrocin reached 52.9%-88.0% ; the drug sensitive rate of E. coli against to ampicillin, tetracycline, and piperacillin was higher than others; the drug sensitive rate of Uu against ciprofloxaein （CPFX） was up to 63.6%. CONCLUSION Bacteria, Uu and CT are the main pathogens causing chronic prostatitis occurred in reproductive stage, and part of them is from the flora in female genital tract. Since bacteria and Uu have developed certain of drug resistance against to antibiotic, it is important to pay close attention to cross infection between couples and to the drug resist- ance, so as to increase the curative ratio of clinical therapy.