中文摘要：

本研究通过在NCBI公共数据库的高梁全基因序列中进行简单重复序列SSR搜索，结果显示共有87950个SSR位点，共有1134种重复，其中二元碱基和三元碱基重复所占比例最大，分别占43．36％，32．38％。根据SSR搜索结果设计了高粱SSR引物，通过两次e—PCR验证和筛选引物，随机合成了50对引物。以先锋和乐食两个高丹草品种为材料进行引物的筛选和评价。结果表明，50对引物中有46对引物出带，但只有8对引物具有多态性。这说明在高梁非编码区设计的引物多态性并不高于EST—SSR引物，但对高梁的SSR引物的开发可以起到补充和促进作用。

英文摘要：

The complete genome sequence of sorghum which downloaded from the NCBI public databases was used for a simple sequence repeated SSR search. The result showed that there were 87 950 SSR loci and 1 134 kinds of repetition about the results in all. The proportion of dinucleotide and trinucleotide repeats was placed on the first place, accounting for 43.36% and 32.38% of the all. SSR primers of sorghum were designed ba- sing on the search results of SSR, according to use the e-PCR to validate and screen primers two times ,50 pairs of primers were arbitrarily selected and synthesized to evaluate these primers by sorghum-sudangrass hybrids such as Xian-Feng and Le-Shi. The results showed that there were 46 target bands among 50 pair primer, and the target bands were characterized clearly and steadily, of which only 8 pairs had polymorphism. It indicated that the polymorphism of sorghum designed primers in non-coding region was not higher than the EST-SSR primers, but it played a complementary and catalytic role on the development of SSR primers in sorghum.