中文摘要：

目的探讨健骨颗粒对成骨细胞中ERα介导的TERT信号通路的调控作用。方法采用雌激素受体拮抗剂ICI182780（Faslodex）阻断成骨细胞中雌激素受体α（ERα）的表达,建立ER抑制的大鼠成骨细胞株ROS1728细胞模型,采用酶联免疫吸附法（ELISA）检测成骨细胞液中碱性磷酸酶（alkaline phosphatase,ALP）、骨钙素（osteocalcin,BGP）、Ⅰ型胶原（collagen I,ColⅠ）的含量。采用实时荧光定量SYBR GREEN法检测ERE、ERα、c-MYCmRNA的表达。采用Western Blot检测TERT、ERα、cMYC蛋白的表达。结果 ELISA法检测结果显示：随着干预时间的延长,培养液中的ALP、BGP、ColⅠ的含量逐渐上升。其中对照组3种信号因子的含量最高血,雌激素组次之,健骨颗粒组再次之,模型组最低,各组比较差异有统计学意义（P〈0.05）,4组TERT、ERα、c-MYCmRNA及蛋白表达量情况以对照组的蛋白表达含量最高,雌激素组次之,健骨颗粒组再次之,模型组最低。各组比较均有统计学意义（P〈0.05）。结论雌激素介导TERT信号通路及其相关因子与成骨细胞分化的关系密切,而补肾健脾中药健骨颗粒可通过雌激素介导TERT信号通路促进成骨细胞分化。

英文摘要：

Objective To investigate the effect of Strong-bone granules on the regulation of TERT signaling pathway mediated by ER alpha in osteoblasts. Methods The estrogen receptor antagonist ICI182780（ Faslodex） was used to inhibit the expression of ER in osteoblasts and to establish the ER-silenced model of rat osteoblast cell line ROS1728. Serum ALP,BGP,and Col I were determined using enzyme-linked immunosorbent assay. The mRNA expression of ERE,ERα,and c-MYC was determined using real time quantitative SYBR GREEN assay. The protein expression of ERE,ERα,and c-MYC was detected using Western blotting.Results The results of ELISA showed that the content of ALP,BGP,and Col in the culture medium increased gradually with the prolonging of intervention time. The levels were the highest in the control group,then followed in estrogen group,Strong-bone granules group,and the model group,and the difference among the groups was significant（ P 〈 0. 05）. The mRNA and protein expression of TERT,ER alpha,and c-MYC was the highest in the control group,then followed in estrogen group,Strong-bone granules group,and the model group,and the difference among the groups was significant（ P 〈 0. 05）. Conclusion The estrogen mediated TERT signaling pathway and its related factors are closely related to the differentiation of osteoblasts. Strong-bone granules promote osteoblast differentiation through estrogen mediated TERT signaling pathway.