中文摘要：

目的：观察川芎嗪（Lig）对心肌细胞膜钙通道及胞内游离Ca^2＋浓度（[Ca^2＋]i。的影响，并探讨其作用机制。方法：急性分离大鼠心肌细胞，用特异性Ca^2＋荧光探针Fluo-3／AM负载细胞，激光共聚焦显微镜（LSCM）检测Lig作用后心肌细胞内[Ca^2＋]i，的变化。结果：Lig（30μmol／L）可降低细胞内[Ca^2＋]i；L-型钙通道阻断剂Verapamil（40μmol／L）、β1受体特平阻断剂Atenolol（900μmol／L）和α1受体特异阻断剂Phentolamine（1×10^-5mol/L）预处理，均可不同程度地阻断Lig（30μmol／L）对心肌细胞钙的作用；Lig（30μmol／L）对α1受体特异激动剂Phenylephrine（1×10^-4mol／L）、钙通道激动剂A23187（900μmol／L）和β1受体特异激动剂Dobutamine（1×10^-5mol／L）引起的细胞内钙升高有抑制作用。不与血管紧张素Ⅱ的Ⅰ型受体（AT1R）阻断剂Valsartan位点作用。结论：Lig对心肌细胞内[Ca^2＋]i。的降低作用，是由细胞膜上的L-型钙离子通道、α1受体和β1受体介导的。

英文摘要：

The purpose of this study was to observe the effect of Ligustrazine （Lig） on the intracellular free Ca^2＋ concentration （[Ca^2＋]i） of cardiac muscle and calcium channels of cell membrane, and investigate its mechanism. The acute-separated ventricular cells of rat with Fluo-3/AM and use laser confocal microscope （LSCM） measure the change of Intracellular free Ca^2＋ concentration [Ca^2＋ ]i. The result showed that Lig （30 μmol＆ #t 8226; L^-1） decreased intracellular free Ca^2＋ concentration. Verapamil （40μmol＆# 8226 ;L^-1） and Phentolamine （1×10^-5mol＆# 8226;L^-1） could partly abolish Lig-induced[Ca^2＋]i decrease. Lig （30 μmol＆# 8226; L^-1） could obviously inhibit the elevation of intracellular free Ca^2＋ concentration which is induced by Phenylephrine （1×10^-4mol＆# 8226 ; L^-1 ）, A32187 （900 μmol＆# 8226 ; L^-1 ） and Dobutamine （1×10^5mol＆# 8226;L^-1）. it has no effect to the effect binding site of AT-1R inhibitor Valsartan. Comclusion Lig decreased intracellular [Ca^2＋ ]i of cardiac muscle by inhibiting α1-receptor, β1-receptor and L-type calcium channels of cell membrane. The relationship among them was to be approved.