中文摘要：

蛋白质的位点特异性修饰近年来取得了重要进展.本文对该领域新近发展的利用高张力烯烃或炔烃与四嗪类化合物的Diels-Alder生物正交反应,通过基因编码的方式在蛋白质中位点特异性地插入其中一个组分,从而实现蛋白质的快速荧光标记进行了介绍.

英文摘要：

In recent years,many achievements have been made in site-specific protein modifications.This review highlights the latest advances on protein labeling,which utilizes the inverse electron-demand Diels-Alder bioorthogonal reactions of highly strained alkenes or alkynes and tetrazines,by genetically encoding unnatural amino acids containing one of these functionalities into the specific sites of proteins,leading to the rapid fluorescence labeling of cellular proteins.