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茶叶多糖的纯化及其光谱特性研究

期刊名称：食品科学. 2007, 28(11):39-43
时间：0
分类：R284.1[医药卫生—中药学;医药卫生—中医学] R284.2[医药卫生—中药学;医药卫生—中医学]
作者机构：[1]南昌大学食品科学教育部重点实验率,江西南昌330047
相关基金：国家自然科学基金资助项目（20462005）;教育部长江学者和创新凼队发展计划资助项目（IRT0540）;南昌大学测试基金资助项目（2005002）
相关项目：茶多糖的一级结构及其在溶液中构象的分子模型

关键词：茶叶多糖, 纯化, 高效凝胶渗透色谱法, 紫外光谱, 红外光谱, tea polysaccharides, purification, high performance gel permeation chromatography （HPGPC）, UV spectra, IR spectra

中文摘要：

利用AKTAPurifier100生物大分子纯化系统纯化茶叶多糖,并采用高效凝胶渗透色谱鉴定其纯度并对其进行紫外和红外光谱分析。结果发现,纯化最佳条件为：上样量5ml,超纯水洗脱,流速2.6ml/min。该方法简便,效率高,能非常方便地放大生产。纯化所得茶叶多糖的多糖衍生物吸收峰和蛋白质特征吸收峰的最大值正好重叠在一起,高效凝胶渗透色谱检测也发现其色谱峰为单一对称色谱峰,紫外280nm和示差检测的出峰时间相差很小,纯度大于99%,表明该均一组分可能是糖蛋白缀合物。同时,紫外和红外光谱分析进一步揭示纯化所得茶叶多糖为一糖蛋白复合物,并提供了一些结构信息。

英文摘要：

Tea polysaccharide was extracted and purified from coarse old tea leaves that were sampled from Shangyou county of Jiangxi Province in China with the AKTA Purifier 100 biological macromolecule purified system. Its purity was identified by the high performance gel permeation chromatography （HPGPC）. The results showed that the optimum purification technical parameters are： the sampling volume of 5 ml under the flow speed of 2.6 ml/min. The results demonstrated that this method is fast, simple, more effective and fit to be magnified. Then it was that its maximum polysaccharide and protein absorption are overlapped, implying that this component is a glycoprotein. HPGPC indicated that it is a homogeneous glycoprotein component and its purity exceeds 99%. At the same time, UV spectra and IR spectra also indicated that tea polysaccharide is a glycoprotein offering much information about its structure.