中文摘要：

目的观察β趋化因子对小鼠小胶质细胞的活化及诱导感光细胞凋亡的作用。设计实验性研究。研究对象β趋化因子、小鼠BV-2小胶质细胞系及661w感光细胞系。方法将β趋化因子（RANTES、MIP-1α及MIP-1β）加入到BV-2细胞中,观察BV-2细胞活化反应。然后将被激活的BV-2细胞培养液上清加入661w细胞中,观察661w的凋亡情况。或将β趋化因子直接加入到661w细胞中,观察其凋亡情况。事先加入β趋化因子抑制剂met-RANTES后,重复上述操作。主要指标钙内流、ROS及NO的产生、TUNEL阳性感光细胞百分比。结果β趋化因子加入BV-2细胞后,细胞内钙流曲线明显上升、细胞外ROS及NO产生较对照组均明显增高（P〈0.01）;将激活后的BV-2细胞上清加入到661w细胞中,后者凋亡率增加30%。β趋化因子直接加入661w细胞中,上述指标无明显变化。在加入β趋化因子之前先加入其抑制剂met-RANTES,BV-2细胞的ROS产生较未加抑制剂组明显降低（P〈0.01）、NO产生较未加抑制剂组降低（P=0.0187）,661w细胞凋亡明显减少。结论β趋化因子可活化小鼠小胶质细胞导致感光细胞凋亡。β趋化因子抑制剂可抑制遗传性视网膜变性小鼠小胶质细胞活化,保护感光细胞。

英文摘要：

Objective To observe the activation of microglia and the photoreceptor cell apoptosis of mice induced byβ-chemokines in vitro. Design Experimental study. Participants β-chemokine, BV-2 cells of mice, 661 w cells of mice. Methods Activation of BV-2 cells were studied when β-chemokines（RANTES, MIP-1αand MIP-1β） were added. The cell death of 661 w cells was observed when the supernatant of the activated BV-2 cells was added into the 661 w cells. The 661 w cell apoptosis was also studied when the β-chemokines were directly added. Met-RANTES（the inhibitor of β-chemokine） was added before hand and above markers were studied. Main Outcome Measures Calcium influx, ROS, NO, TUNEL positive cells. Results The calcium influxesas well as release of ROS, NO by the BV-2 cells was increased（P〈0.01） after adding β-chemokine.The 661 w cell death was elevated by 30% after adding the supernatant of activated microglia. Above markers can be inhibited by the β-chemokine inhibitors（ROS P〈0.01, NO P=0.0187）.There was no change when adding β-chemokine into 661 w cells directly. Conclusions β-chemokines can activate microglia,which induce photoreceptor cell death by release of ROS or NO. Photoreceptor degeneration of RP mice may be protected by inhibition of β-chemokine inhibitors.