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中文摘要：
利用超高效液相色谱-质谱联用（UPLC-MS）技术结合主成分分析方法研究制川乌单煎液、制川乌与白芍、制川乌与防己共煎液在大鼠肠内菌中的代谢差异。采用SIMCA-P软件，以肠内菌代谢后乌头类生物碱的相对含量为变量进行主成分（PCA）分析。在主成分得分图中，制川乌单煎液与制川乌-白芍、制川乌-防己共煎液均可以明显区分，说明制川乌单煎液与制川乌-白芍、制川乌-防己共煎液的肠内菌生物转化存在显著差异。通过主成分分析载荷图及独立样本t检验，从制川乌-白芍组得到7种差异显著的标志物，从制川乌-防己组得到6种标志物，其中制川乌-白芍组有4种标志物经肠内菌代谢后含量高于制川乌组，而制川乌-防己组有1种化合物含量高于制川乌组，两组中其它标志物含量低于制川乌组。这些标志物可能是制川乌配伍前后药效差异的物质基础。
英文摘要：
The ultra performance liquid chromatography coupled with mass spectrometry（UPLC-MS）was used to investigate the metabolic difference of the decoction of Radix Aconiti Preparata （ RAP ） and its co-decoctions with Radix Paeoniae Alba （ RAP-RPA ） or Radix Stephaniae Tetrandrae （ RAP-RST ） in rat intestinal bacteria. The principal component analysis （ PCA） of the relative contents of Aconitum alkaloids after metabolism was performed by SIMCA-P software. The score plots of PCA could successfully distinguish the three groups of RAP, RAP-RPA and RAP-RST. The result indicated that the differences of biotransformation among the groups of PAP, RAP-RPA and RAP-RST were significant. With the loading plot and independent-samples T test, seven relevant markers with the significant differences were found in the group of RAP-RPA, six relevant markers were obtained in the group of RAP-RST. The relative content of four markers in RAP-RPA was higher than that in RAP, and one marker in RAP-RST was higher than that in RAP. The relative contents of other markers were all lower than that in RAP. These markers may be the effective substance for explaining the different effects of Radix Aconiti Preparata before and after combination with Radix Paeoniae Alba and Radix Stephaniae Tetrandrae.
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