中文摘要：

目的：探讨miR-338基因簇在食管癌组织中的表达模式及其与食管癌发病风险的关系。方法：选取86例经病理确诊的食管癌患者,分别提取食管癌和癌旁组织总RNA,采用实时荧光定量PCR法检测hsa-miR-338-3p、hsa-miR-338-5p与hsa-miR-657的表达,应用配对样本t检验分析癌和癌旁组织中miRNA表达差异,Pearson相关分析检验基因簇各miRNA表达的相关性,logistic回归分析miRNA异常表达对食管癌发病风险的影响。结果：hsa-miR-338-3p与hsa-miR-338-5p在食管癌组织中的表达均较癌旁组织降低（P均〈0.05）,hsa-miR-657在食管癌和癌旁组织中表达的差异无统计学意义（P〉0.05）,hsa-miR-338-3p与hsa-miR-338-5p表达显著相关（r=0.754,P〈0.05）,hsa-miR-338-5p的低表达增加了食管癌的发病风险（OR=1.264,P〈0.05）,可能是食管癌的危险因素。结论：miR-338基因簇可能抑制了食管癌的发生,hsa-miR-338-5p可能成为食管癌诊断的潜在标志物。

英文摘要：

OBJECTIVE： To investigate the expression level of miR-338 cluster in esophageal carcinoma and identify the relationship between miR-338 cluster and esophageal carcinoma. METHODS：A total of 86 cases of patients with confirmed esophageal carcinoma were selected,real time RT-PCR were performed to detect the expression of hsa-miR-338-3p,hsa-miR-338-5p and hsa-miR-657. We used paired t-test to analyze the expression of miRNA in tumor and adjacent non-tumor tissues,Pearson correlation analysis to examine the correlation of the expression of each miRNA and logistic regression a nalysis to analyze t he e ffect of a bnormal expression of miRNAs on the risk of esophageal cancer. RESULTS：hsamiR-338-3p and hsa-miR-338-5p were downregulated in tumor tissues compared with adjacent non-tumor tissues（P〈0.05）,and no statical difference was found of the expression of hsa-miR-657 between the two tissues（P〉0.05）. The expression of hsa-miR-338-3p and hsa-miR-338-5p were significantly correlated（r=0.754,P〈0.05）,and low expression of hsa-miR-338-5p was the risk factor for esophageal carcinoma（OR=1.264,P〈0.05）. CONCLUSION：miR-338 cluster may play an important role in the formation process of esophageal carcinoma. In addition hsa-miR-338-5p may represent the miR-338 cluster to be a useful biomarker for diagnosis.