中文摘要：

目的探讨肝癌射频消融（radiofrequency ablation，RFA）术后肿瘤血管生成在残留癌进展中的作用。方法建立兔VX2肝癌模型，以不同温度（55℃、70℃和85℃）行RFA，建立肝癌RFA后残留癌模型，对照组未行RFA。通过大体病理观察不同消融温度对残留癌局部生长和肺转移的影响。采用免疫组化法检测残留癌组织MVD、VEGF的表达，Westernblot法检测残留癌组织VEGF蛋白表达，RT-PCR法检测残留癌组织VEGFmRNA表达。结果肝癌射频消融术后与对照组（9．91±0．98）cm’比较，各RFA处理组局部肿瘤体积明显增大（分别江-17．43，-10．1l，-8．79，均P〈0．05）；与70℃组（17．08±2．28）cm’和85℃组（15．95±4．95）cm’比较，55cC组残留癌体积（21．26±2．32）cm。增大更为明显（分别t=4．69，6．78，均P〈0．05）。各RFA处理组肺转移程度较对照组显著增强，并以55℃处理组最为显著（分别f=-21．65，-30．15，均P〈0．05）。免疫组化法显示，与对照组比较，各RFA处理组残留癌组织MVD、VEGF表达均明显增强（MVD分别t=-13．01，-5．46，-5．63，均P〈0．05），（VEGF分别t=8．00，4．92，4．21，均P〈0．05）；其中，与70℃组和85℃组比较，55cc组更为显著（MVD分别t=5．41，6．15，均P〈0．05）（VEGF分别t=2．45，4．10，均P〈0．05）。各RFA处理组残留癌组织VEGF蛋白和VEGFmRNA表达水平均高于对照组。结论VEGF过表达，加速肿瘤血管生成是肝癌RFA后残留痛怏涑进展的机制之一。

英文摘要：

Objective To explore experimently the effect of tumor angiogenesis on rapid progression of residual tumor of liver cancer after radiofrequency ablation （RFA）. Methods A rabbit VX2 hepatoma model was established. Inoculated tumors were treated by using RFA at 55 ℃, 70 ℃ and 85 ℃ respectively to establish the residual VX2 hepatoma model. Rabbits implanted with VX2 hepatoma but receiving no RFA treatment served as controls. The expression of vascular endothelial growth factor （VEGF） was determined in tumors to assess the relationship between VEGF and the focal tumor volume and distant metastasis. The expression of VEGF and mierovessel density （MVD） in tumor tissues was assessed by immunohistochemistry. The protein expression of VEGF was assessed by Western blot. The expression of VEGF mRNA was detected by RT-PCR. Results There were significant differences of the local tumor volume between the control group （ 9.91 ± O. 98 ） cm^3 and the other groups （ respectively t = - 17.43, - 10. 11, - 8. 79, all P 〈 0. 05）. Compared with the 70 ℃ group （ 17.08 ±2. 28 ） cm^3 and the 85 ℃ group （15.95±4.95） cm^3,the focal tumor volume of 55 ℃ group was the largest （21.26 ±.32） cm^3, （ respectively t = 4. 69,6. 78, all P 〈 0.05 ）. Much more metastatic lesions of lung were observed in the RFA treated groups in comparison to the control group. Moreover, the lung metastasis in 55 ℃ group was the most serious among the three RFA treated groups （ respectively t = - 21.65, - 30. 15, all P 〈 0. 05 ）. Immunohistochemieal staining indicated that the exoression of VEGF and MVD in the RFA treated groups was much higher than those in control group （ MVD respectively t = - 13.01, - 5.46, - 5.63, all P 〈 0. 05）, （ VEGF respectively t = 8.00,4. 92, d. 21, all P 〈 0.05 ）. Furthermore, the expression of both VEGF protein and VEGF mRNA in 55 ℃ group was the highest among the three RFA treated groups. Conclusions The over-expression of VEGF accelerating the tumor angiogenes