中文摘要：

目的：探讨小鼠诱导性多能干细胞IP14D-1是否具备诱导性原始生殖细胞（induced primordial germcells,iPGCs）分化潜能,以及特异基因表达变化及可能机制。方法：未分化IP14D-1培养扩增,分化形成诱导性拟胚体（induced embryoid bodies,iEBs）。RT-PCR和免疫荧光分别检测4、7、9 d的iEBs中Lin28、Blimp1、Stra8和Mvh的表达变化和蛋白定位情况。结果：未分化IP14D-1同小鼠胚胎干细胞（mouse embryonic stem cells,mESCs）相同,Lin28表达较弱,Blimp1表达相对较强,Mvh和Stra8也在这两种细胞及其相应iEBs和EBs中表达,但均无明显差别。IP14D-1分化形成的iEBs从4 d生长到7 d时,Lin28表达逐渐增强,到9 d时表现为下降,Blimp1表达则随着iEB生长时间延长而逐渐降低。结论：建立了IP14D-1和相应拟胚体（iEBs）的完善、稳定的培养及分化体系;未分化IP14D-1与mESCs在Lin28、Blimp1、Mvh和Stra8表达方面无明显差别;iEB和EBs的Mvh和Stra8表达也无明显差别。IP14D-1及iEBs具有iPGCs分化潜能,且可能是7 d的iEBs内iPGCs分化数量较多,之后进入iPGCs分化的Lin28低表达时期。

英文摘要：

Objective： To investigate whether mouse-induced pluripotent stem（iPS） cell line IP14D-1 has the potential to differentiate into induced primordial germ cells（iPGCs）,and to explore the changes in the expression of iPGCs-differentiation associated genes and their possible mechanisms.Methods： Undifferentiated IP14D-1 was cultured to proliferate and then differentiated to form 4-,7-and 9-day-old induced embryoid bodies（iEBs） in vitro,respectively.RT-PCR and immunofluorescence were used to detect the expressions of Lin28,Blimp1,Stra8 and Mvh,as well as the localization of the corresponding protein in iEBs.Results： The expression of Blimp1 was higher than that of Lin28 in the undifferentiated IP14D-1 and mouse embryonic stem cells（mESCs）.Mvh and Stra8 as well as mESCs and EBs were also expressed in IP14D-1 and iEBs,but with no significant differences.The expression of Lin28 was gradually increased in the IP14D-1-derived iEBs from 4 to 7 days,but decreased at 9 days,and the expression of Blimp1 was gra-dually reduced with the prolonged growing time of iEBs.Conclusion： A stable system was established for the culture and differentiation of IP14D-1 and IP14D-1-derived iEBs.The expressions of Lin28,Blimp1,Mvh and Stra8 were not significantly different between the undifferentiated IP14D-1 and mESCs,nor were the expressions of Mvh and Stra8 between iEBs and EBs.IP14D-1 and iEBs had the potential to differentiate into iPGCs,which increased in number in the 7-day-old iEBs,and the expression of iPGC-differentiation associated Lin28 became lower in the older iEBs.