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中文摘要：
在水温（20.0±0.2）℃条件下,采用口灌方法,研究了不同用药剂量的甲砜霉素对体质量为（82.30±13.59）g的松浦镜鲤肝胰脏的毒性作用。分别选取1、3、5倍的正常用药推荐剂量,并将用药期设为3倍剂量的最大推荐使用期限,即以0、50、150、250 mg/kg（体质量）的剂量对试验鱼连续口灌甲砜霉素15 d,每天一次,并设口灌蒸馏水组为对照组,于停药24 h时采集各组试验鱼的肝胰脏组织,测定试验鱼肝胰脏中过氧化氢酶（CAT）、超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量,并对其组织切片进行观察。结果表明：随着用药剂量的增加,试验鱼肝胰脏中SOD和CAT活性表现为先激发后抑制的趋势,SOD和CAT活性在1倍用药剂量时,达到峰值;随着用药剂量的增加,试验鱼肝胰脏中MDA含量先降低后升高,在1倍用药剂量时,MDA含量为最低,高剂量用药组肝胰脏中MDA含量显著高于对照组（P〈0.05）。组织切片观察表明,随着用药剂量的增加,试验鱼肝胰脏组织受到的损伤加重。研究表明,甲砜霉素对松浦镜鲤的肝胰脏具有一定的毒性,且随着用药剂量的增加毒性急剧增大,甲砜霉素的用药剂量对松浦镜鲤肝胰脏的免疫功能也有一定影响。
英文摘要：
The toxicity of thiamphenicol [0（ as control group）,50,150,250 mg/kg body weight,5 times as high as toxic dose]to hepatopancreas of Songpu mirror carp Cyprinus carpio Songpu（ body weight 82.30 g ± 13.59 g）was studied at water temperature of（ 20.0 ± 0.2） ℃ by daily oral administration for 15 days.The activities of catalase（ CAT）,and superoxide dismutase（ SOD） and malondialdehyde content in hepatopancreas were determined and histological observation of the hepatopancreas was conducted 24 h after stopping oral administration.Results showed that the activities of CAT,and SOD were found a trend of first enhancement and then decline,the peak in 50mg/kg group,and the content of MDA showed a trend of first decline and then enhancement with the increase in thiamphenicol dosage,the minimum in 50 mg/kg group.There was significantly higher MDA content in high doses group than that in the control group.The histological observation showed that the hepatopancreas damage was aggravating with the increase in the drug dosage,indicating that thiamphenicol has certain dose-dependant toxicity,and certain effect on immunity in Songpu mirror carp.So we should pay more attention to thiamphenicol dosage in use.
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