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中文摘要：
以黄瓜无侧枝品系419（P1）和有侧枝品系SB-2（P2）为亲本构建的136株F2群体为试验材料,对黄瓜无侧枝基因nlb进行定位研究。田间调查和遗传分析结果表明,F2群体的表现型中有侧枝与无侧枝的分离比为3︰1。运用分离群体分组混合分析法（bulked segregant analysis,BSA）,从F2无侧枝群体和有侧枝群体中各随机选取10株,分别混合其DNA,构建无侧枝和有侧枝基因池,通过分析SSR分子标记在基因池间的多态性,筛选得到nlb基因连锁标记9个。然后利用F2群体进行进一步验证,经MAPMAKER3.0软件分析,将nlb基因定位于黄瓜1号染色体,其中距离nlb基因较为紧密的标记是SSR19673,遗传距离为15.9cM。
英文摘要：
A F2 population including 136 individuals derived from the crossing of non-lateral branch line 419（P 1 ）and branch line SB-2（P 2 ）,using this F2 population as materials to research on non-lateral branch（nlb）gene mapping of cucumber. We found that F2 population may be divided into lateral branch individuals and none lateral branch individuals by field investigation and genetic analysis,and their proportion fit for 3：1. Constructing none branch and branch gene pools by taking bulked segregant analysis（BSA）which select 10 individuals from F2 non-branch population and branch population randomly and mix their DNA respectively. We found 9 simple sequence repeat（SSR）markers which have polymorphism between two gene pools was linked to nlb gene. The F2 population was used on further verification and found molecular marker SSR19673 which is closer to nlb gene with genetic distance 15.9 cM through analysis of software MAPMAKER 3.0,therefore,nlb gene was mapped on the first chromosome of cucumber.
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