中文摘要：

研究几种常见铁化合物的铁有效性和毒性。用1.5 mmol/L的各种铁化合物溶液分别孵育Caco-2细胞24 h后,用细胞铁吸收量作为铁有效性指标,通过3-（4,5）-二甲基-2-噻唑-（2,5）-二苯基溴化四氮唑蓝（MTT）比色、乳酸脱氢酶（LDH）渗漏及细胞碱性磷酸酶（AKP）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性分析评价铁对细胞的毒性。EDTA-FeNa铁有效性显著高于其他铁化合物（P〈0.05）。MTT和LDH分析表明,EDTA-FeNa对细胞毒性最大;几种铁强化剂均显著降低了细胞AKP活性,FeC l3对SOD的消耗显著高于其他铁化合物（P〈0.05）,1.5 mmol/L EDTA-FeNa和柠檬酸亚铁可诱导细胞内GSH-Px的产生。1.5mmol/L铁浓度对Caco-2细胞已构成毒害,且二价铁毒性大于三价铁;铁有效性越高的化合物,其毒性越强,本研究中EDTA-FeNa铁有效性和毒性均显著高于其他铁化合物。

英文摘要：

The objective of the present study is to determine the availability and toxicity of different iron compounds in a human intestinal cell line,Caco-2.Caco-2 cells were incubated with 1.5 mmol/L of iron for 24 h,thereafter toxicological and uptake experiments were performed.The iron uptake was measured by intracellular iron concentration.The cellular damage was measured by using MTT assay,lactate dehydrogenase（LDH）-release.Furthermore,the activities of major antioxidative enzymes [superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px）]and differentiation marker [alkaline phosphatase（ALK）]of Caco-2 cells incubated with various iron compounds differed significantly from untreated control which showed no detrimental effects on cells and less iron uptake.The highest intracellular iron concentration was detected after the treatment of Caco-2 cells with EDTA-FeNa（P0.05）.The lowest signal of MTT assay and the highest signal of LDH-release were found after the incubation of the cells with EDTA-FeNa.The ALK activity of the cells treated with different iron compounds was significantly decreased than untreated control（P0.05）.The SOD activity of the cells treated with FeCl3 was significantly decreased than those of the cells treated with other iron compounds（P0.05）.EDTA-FeNa and ferrous citrate could induce the cellular GSH-Px exudation at 1.5 mmol/L iron.The Caco-2 cells were damaged by various iron forms,which showed significantly detrimental effect from Fe（II） than from Fe（III） at 1.5 mmol/L iron level.In this study,the highest availability and toxicity of iron were found after the incubation of the cells with EDTA-FeNa.We suggest that the toxicity of iron on cells show positive and significant correlation with iron bioavailability of iron compounds.