中文摘要：

目的摸索并建立表皮干细胞Oct4基因的非转基因诱导方法。方法采用RT-PCR方法 ,检测经6-氨基己酸刺激48h后表皮干细胞Oct4基因的转录水平。结果 6-氨基己酸能有效激发表皮干细胞Oct4基因的转录。结论 RT-PCR方法检测表皮干细胞Oct4基因转录水平变化的准确度高、重复性好,实验步骤简单,是初步筛选诱导基因转录表达小分子化合物的实用方法 。

英文摘要：

Objective To develop the non-transgenic method for inducing transcription of oct4 gene in epidermic stem cells.Methods RT-PCR was performed to measure the transcription level of oct4 gene in epidermic stem cells 48h after activated by 6-aminohexanoic acid.Results 6-aminohexanoic acid effectively activated the transcription of oct4 in epidermic stem cells.Conclusion RT-PCR can accurately and repeatedly detect the transcription level of oct4 gene in epidermic stem cells,and is thus a simple and practical method for screening the potential small molecular compounds expressed in inducing transcription genes.