中文摘要：

把转的效率和一次和连续调停的杀死的效果与 GE7 作比较的目的，一个非病毒的指向的交货系统，在进卵巢的癌症房间的单纯性疱疹病毒(HSV-tk ) 的胸腺嘧啶核甙激酶基因的 transfection。方法 GE7 被用来与 β-galactosidase (β-gal ) 和 HSV1-tk 准备 recombinants；recombinants 然后习惯于 transfect 人的卵巢的癌症线 CaOV3 一次并且连续地。染色的 β-gal 被用来把一次和连续调停的效率与 GE7 系统作比较。Ganciclovior (GCV ) 被介绍进 HSV1-tk transfected 卵巢的房间。通过画房间生长曲线和流动血细胞计数，杀死 GCV 完成在上曾经并且连续地， GE7/HSV1-tk transfected 房间被观察。我们发现了的结果一次和连续外长的基因转移效率，这分别地是大约 80% 和 85% 。当 1 μg/mL GCV 被用来与 HSV1-tk 基因对待卵巢的细胞 transfected 时，禁止的生长一次并且连续的转的卵巢的细胞评价分别地是 82% 和 90% ；他们的 apoptosis 索引分别地是 15 和 30。在一样的 GCV 集中下面，进卵巢的癌症房间的 GE7/pCMV-tk transfection 的连续调停比一次调停有更多的重要禁止的效果(P 【 0.05 ) 。结论与一个相比预定调停，有 GE7 基因交货系统的 transfection 的连续调停有更高的效率。GE7/HSV1-tk/GCV 治疗学的基因系统的连续调停有更多的强大的杀死效果。

英文摘要：

Objective： To compare the transferring efficiency and killing effects of one time and continuous mediation with GE7, a non-viral targeted delivery system, in transfection of thymidine kinase gene of herpes simplex virus （HSV-tk） into ovarian cancer cells. Methods： GE7 was used to prepare recombinants with β-galactosidase （β-gal） and HSVI-tk; the recombinants were then used to transfect human ovarian cancer line CaOV3 once and continuously. β-gal staining was used to compare the efficiencies of one time and continuous mediation with GE7 system. Ganciclovior （GCV） was introduced into HSVI-tk transfected ovarian cells. Through drawing the cell growth curve and flow cytometry, the killing effects of GCV on once and continuously GE7/HSVI-tk transfected cells were observed. Results： We found that the one time and continuous exogenous gene transfer efficiencies were about 80% and 85%, respectively. When 1 μg/mL GCV was used to treat ovarian cell transfected with HSVI-tk gene, growth inhibiting rates of ovarian cells of one time and continuous transferring were 82% and 90%, respectively; their apoptosis indices were 15 and 30, respectively. Under same GCV concentration, continuous mediation of GE7/pCMV-tk transfection into ovarian cancer cells had more significant inhibitory effect than one time mediation （P 〈 0.05）. Conclusion： Compared with one time mediation, continuous mediation of transfection with GE7 gene delivery system has higher efficiency. Continuous mediation of GE7/HSVI-tk/GCV therapeutic gene system has more powerful killing effect.