中文摘要：

为评估转cry1Ab/cry2Aj、cry1Ab/vip3DA玉米对棉铃虫Helicoverpa armigera（Hübner）、甜菜夜蛾Spodoptera exigua（Hübner）和斜纹夜蛾Prodenia litura（Fabricius）的抗虫性,在室内测定了3个转cry1Ab/cry2Aj玉米品系和1个转cry1Ab/vip3DA玉米品系对3种害虫幼虫存活和生长发育的影响,研究了该系列Bt玉米不同组织器官对害虫的杀虫活性和控制效果。结果显示,棉铃虫初孵幼虫取食各品系Bt玉米叶片96h后死亡率为87.50%~90.00%,取食花丝和雌穗的幼虫96h后几乎全部死亡;甜菜夜蛾初孵幼虫取食各品系Bt玉米叶片、花丝和雌穗168h后死亡率为22.50%~68.33%,幼虫的生长发育受到明显抑制,体重抑制率达85.00%~95.00%;斜纹夜蛾初孵幼虫取食各品系Bt玉米叶片、花丝和雌穗96 h后死亡率显著高于非转基因亲本对照,168h后幼虫死亡率达90.00%以上。研究表明,转cry1Ab/cry2Aj和cry1Ab/vip3DA玉米品系对棉铃虫和斜纹夜蛾的初孵幼虫表现出较好的抗性,可以作为转多基因抗虫玉米育种的备选材料。

英文摘要：

In order to evaluate the resistance of transgenic cry1Ab/cry2Aj maize and cry1Ab/vip3DA maize to Helicoverpa armigera（Hübner）,Spodoptera exigua（Hübner） and Prodenia litura（Fabricius）,the survival and development of the larvae of the three insect pests treated with three transgenic cry1Ab/cry2Aj maize events and one transgenic cry1Ab/vip3DA maize event were conducted through laboratory bioassay,and the insecticidal activity and control efficacy of different tissues and organs of these Bt events were investigated.The results showed that the mortalities of H.armigera neonate larvae were 87.50%-90.00%when fed on leaves of Bt maize events at 96 h after infestation,and most larvae fed on silks and ear of Bt maize events died at 96 h after infestation.The mortalities of S.exigua neonate larvae fed on leaves,silks and ear of Bt maize events were 22.50%-68.33%at 168 h after infestation,while the larval development was inhibited severely and the weight inhibition rate ranged from 85.00%to95.00%.The mortalities of P.litura neonate larvae fed on leaves,silks and ear of Bt maize events were significantly higher than that of the control at 96 h after infestation,and more than 90.00%larvae were killed at 168 h after infestation.The results indicated that these transgenic cry1Ab/cry2Aj and cry1Ab/vip3DA maize events provided efficient resistance against H.armigera and P.litura neonate larvae,and could serve as breeding sources for the development of insect-resistant pyramided transgenic maize.