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氮、钾、甜菜碱对水分胁迫下夏玉米叶片膜脂过氧化和保护酶活性的影响
  • ISSN号:0496-3490
  • 期刊名称:《作物学报》
  • 时间:0
  • 分类:S718.43[农业科学—林学]
  • 作者机构:[1]西北农林科技大学生命学院, [2]西北农林科技大学资源环境学院,陕西杨凌712100
  • 相关基金:国家自然科学基金重点项目(30230230);国家自然科学基金农业倾斜项目(30070429);西北农林科技大学人才项目(2005)
中文摘要:

采用盆栽试验研究了水分胁迫和适量供水条件下,氮、钾和甜菜碱对2种不同基因型夏玉米陕单9号(抗旱品种)和陕单911(不抗旱品种)各生育期叶片膜脂过氧化和保护酶活性的影响,旨在揭示这些因子通过提高上述酶活性而增强作物抗旱性的生理功能。结果表明,水分胁迫下夏玉米超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性显著降低,不抗旱品种更甚;而丙二醛(MDA)含量有不同程度升高,抗旱品种的MDA含量和变幅小于不抗旱品种。苗期2个品种对水分胁迫响应较弱,SOD、POD、CAT和MDA均较低;拔节和抽雄期响应强烈,酶活性增高。SOD和POD达到最大值的时间比CAT晚,MDA在全生育期中呈现“升-降-升”的变化规律。施用氮、钾肥和甜菜碱能不同程度地提高夏玉米SOD、POD和CAT活性,降低MDA含量,减缓水分胁迫下膜脂过氧化作用。不抗旱品种施用氮肥增强了水分胁迫条件下叶片SOD、POD、CAT活性,降低了MDA含量,氮肥用量的影响有显著差异;抗旱品种施用低氮效果显著,施用高氮则降低了生育前期酶活性,增加了MDA含量,但生育后期氮肥用量的影响间无显著区别。钾肥和甜菜碱对受水分胁迫的夏玉米表现出比氮肥更突出的效果。而对适量供水条件下的夏玉米,氮、钾肥的作用明显下降,甜菜碱的效果甚至消失。说明施用氮、钾肥和甜菜碱对改善水分胁迫下玉米叶片膜脂过氧化作用和提高保护酶活性有重要贡献。

英文摘要:

A pot experiment was conducted under water stress and adequate water-supplying conditions with two cultivars of maize Shaandan 9 (drought-resistant) and Shaandan 911 (non-drought-resistant) to study the effects of nitrogen, potassium, and glycinebetaine on lipid peroxidation and protective enzyme activities in maize leaves at different growing stages for revealing their biological functions in the rise of crop resistance to drought by raising activities of these protective enzymes. Results showed that under the water stress condition, activities of superoxide dismutase (SOD), catalase (CTA), and peoxidase (POD) in leaves were significantly reduced while malondialdehyde (MDA) content was increased for both cuhivars, but the decline was more obvious for the non-drought resistant one. Activities and variation of SOD, CTA, and POD were higher while MDA content was lower for the drought resistant cultivar (Shaandan 9) than those for the nondrought resistant cultivar (Shaandan 911). The lower activities of SOD, CTA, and POD at seeding stage than those at elongation and heading stages showed that the response of two cultivars to water stress was weaker at earlier than at later stages. Of these protective enzymes, activities of SOD and POD reached their peak later than that of CTA. MDA content followed a changing pattern of "rise-fall-rise" in the entire maize growth period. Applications of nitrogen, potassium, and glycinebetaine raised the activities of SOD, CTA, and POD while decreased the MDA content, and thus alleviated water stress effect. Under the water-stressed condition, addition of N fertilizer significantly increased activities of SOD, CTA, POD and decreased MDA content for the non-drought resistant cultivar with a significant difference among N rates. The significant response of drought resistant cultivar to N addition was merely found at low N rate. For high N rate, activities of SOD, CTA and POD were significantly decreased whereas MDA content was increased at earlier gro

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期刊信息
  • 《作物学报》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学技术协会
  • 主办单位:中国作物学会 中国农业科学院作物科学研究所
  • 主编:万建民
  • 地址:北京海淀区中关村南大街12号中国农业科学院
  • 邮编:100081
  • 邮箱:zwxb301@caas.cn
  • 电话:010-82108548
  • 国际标准刊号:ISSN:0496-3490
  • 国内统一刊号:ISSN:11-1809/S
  • 邮发代号:82-336
  • 获奖情况:
  • 2002年-第三届中国科协优秀科技期刊二等奖,2002-2009年-百种中国杰出学术期刊,2004年获“全国优秀期刊一等奖”,2005年获第三届国家期刊奖提名奖,2009年评为“2008年度中国精品科技期刊”,2009年被评为“新中国60年有影响力的期刊”,2011年获"第二届中国出版政府奖期刊奖提名奖"
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,美国剑桥科学文摘,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),英国食品科技文摘,中国北大核心期刊(2000版)
  • 被引量:49369