中文摘要：

目的 探讨钙离子通道α1在原发性手汗症并腋窝多汗患者腋窝汗腺组织中的表达情况.方法 选择2011年10月至2012年9月在我院确诊为原发性手汗症并腋窝多汗的患者28例和同期正常对照组8例,通过手术时切取患者腋窝皮肤汗腺组织1块,显微镜下观察汗腺组织结构,采用Real time PCR技术和Western blot蛋白印迹技术测定钙离子通道α1在原发性手汗症并腋窝多汗患者腋窝汗腺组织中的表达情况.结果 光镜下两组汗腺组织结构无差异,1.25万倍电镜下可见手汗症组汗腺分泌颗粒数量明显高于对照组,差异有统计学意义（P＆lt;0.05）.原发性手汗症并腋窝多汗患者腋窝汗腺组织中钙离子通道α呈高表达,其亚单位α1 mRNA和蛋白水平均高于对照组,差异有统计学意义（P＆lt;0.05）.结论 原发性手汗症并腋窝多汗患者腋窝汗腺组织中汗腺分泌颗粒数量明显增多,钙离子通道α1表达增高,这些可能是手汗症的发病原因之一.

英文摘要：

Objective To investigate the expression level of the calcium channel α1 m patients with primary palmar nyper- hidrosis along with axillary hyperhidrosis. Methods We choosed 28 patients who were diagnosed as primary palmar hyperhidro- sis along with axillary hyperhidrosis from October 2011 to September 2012 in our hospital and 8 cases of normal control group during this period. When the patients were operated, we cut out a block of axillary skin sweat gland tissue. We observed sweat gland structure by light and electron microscope, and used Real time PCR technology and Western blot protein imprinting tech- nology to determine the expression level of calcium channel al in patients with primary palmar hyperhidrosis along with axillary hyperhidrosis. Results Sweat gland structure had no difference by light microscope. The sweat gland secretion granules of ex- perimental group were obviously higher than that of control group by 12 500 times electron microscopy, The expression levels of calcium channel α1 mRNA and its protein were higher in patients with primary palmar hyperhidrosis along with a axillary hyper- hidrosis than those in normal group, and the difference was statistically significant （P〈0. 05）. Conclusion Sweat gland secre- tion granules obviously increased, the expression level of calcium channel a1 increased in the axillary sweat gland tissues of the patients with primary palmar hyperhidrosis along with axillary hyperhidrosis, these might be one of the causes of primary palmar hyperhidrosis.