中文摘要：

目的探讨骨折愈合过程中牙本质基质蛋白1（dentin matrix protein 1，DMP1）与破骨细胞的时间效应。为研究DMP1在体内矿化重建中的作用提供参考。方法将40只成年Wistar雄性大鼠左侧下颌支骨折，建立下颌骨骨折模型。骨折后5、7、14、21d处死大鼠，取骨痂和对侧正常骨组织（对照组），分别采用HE染色、TRAP染色和免疫组织化学链霉抗生物素．蛋白过氧化物酶（streptavidin perosidase，sp）法染色切片检测。结果在对照组正常下颌支组织中没有DMP1的表达，偶见破骨细胞；实验组在骨折后14～21d是破骨细胞活动高峰。结论DMP1与破骨细胞在骨折愈合过程中具有一定的时间效应。

英文摘要：

Objective To study the expression of dentin matrix protein 1 （ DMP1 ） and osteoclast in callus at different healing period of mandibular fracture in a adult Wistar rat model Methods The mandibular fracture model of Wistar rats at the left mandibular ramus was established. The callus in the fractured site and the normal mandible were amputated at the 5th, 7th, 14th and 21 st day after the fracture. HE staining was used to observe the condition of fracture healing and TRAP staining used to observe the activation of osteoclast. The expression of DMP1 was detected in the callus by using immunohistochemical method. Results The number of osteoclasts reached a peak from the 14th to 21st day. The expression of DMP1 became very active from the 7th to 14th day. Conclusions The expression of DMP1 and osteoclast during fracture healing exhibited time effect.