中文摘要：

目的探讨磷脂酶D1（phospholipase D1,PLD1）mRNA在前列腺癌细胞雄激素非依赖性转化中的作用。方法利用Arraystar Human LncRNA Microarray V3.0基因芯片,比较了前列腺癌标准株细胞LNCa P在发生雄激素非依赖性转化前后的mRNA和lncRNA的差异表达,分析表达显著的PLD1 mRNA及其信号通路的相关基因,检测PLD1 mRNA表达受相应shRNA慢病毒载体抑制后LNCa P细胞增殖能力的改变。结果 LNCa P细胞在雄激素非依赖性转化后其PLD1mRNA升高373倍（P〈0.05）,PI3K/AKT信号途径的有关mRNA表达升高;在其PLD1 mRNA表达受慢病毒抑制后,与空白对照和阴性对照组比较,其生长率下降了近30%,差异均有统计学意义（P〈0.05）。结论 PLD1 mRNA及其有关的PI3K/AKT信号通路在前列腺癌细胞雄激素非依赖性中可能存在重要作用,下调PLD1 mRNA的表达能在一定程度上抑制前列腺癌细胞雄激素非依赖转化后的增殖速度。

英文摘要：

Objective To investigate the role of phospholipase D1（PLD1） mRNA in androgen-independent transformation of prostate cancer cells.Methods We used Arraystar Human LncRNA Microarray V3.0 to compare the differential expression of mRNA and lncRNA between LNCa P and LNCa P-AI cells.Related genes of differentially expressed PLD1 mRNA and its signaling pathway were analyzed.The cell reproductive capacity of LNCa P was detected after expression of PLD1 mRNA was inhibited by shRNA lentiviral vector.Results PLD1 mRNA increased to its 373 times after androgen independent transformation of LNCa P cells,and expression of mRNA in PI3 K/AKT signaling pathway increased.The growth rate of LNCa P-AI cells decreased by nearly 30% compared with the blank control and negative control groups after inhibition of Lentivirus,with the difference statistically significant（P〈0.05）.Conclusion PLD1 mRNA and its related PI3 K/AKT signaling pathway may play an important role in androgen independent prostate cancer cells.Down regulation of PLD1 mRNA can inhibit the proliferation of prostate cancer cells after androgen-independent transformation to a certain extent.