中文摘要：

下一代测序技术凭借其高通量的特点成为现代生命科学研究的重要工具之一。转录组学是功能基因组学研究的重要内容，畜禽转录组学的研究是国内外生命科学研究的热点内容。文章综述了下一代测序技术的原理及特点，转录组学的研究概要，重点介绍了下一代测序技术在畜禽转录组研究中的应用进展，最后对下一代测序技术在生命科学研究中存在的问题和今后的发展方向进行了展望。

英文摘要：

Next generation sequencing techniques are among the most powerful tools in modern science research,and play a key role in genomic sequencing. Transcriptomics is important in functional genomics, and the transcriptomics of livestock and poultry is now a hotspot of life science at home and abroad. This paper reviews the principles and the characteristics of the next generation high-throughput sequencing techniques and research profiles of transcriptomics with a particular focus on the application in the tran- scriptomics of livestoek and poultry. At last,the existing problems and future directions are analyzed and prospected.