中文摘要：

基本成纤维细胞生长因素(bFGF， FGF-2 ) 在鼠科的 C2C12 myoblasts 在 myostatin 基因的表达式上有禁止的效果，出现在我们的最近的调查。为了进一步在 myostatin 基因上验证 bFGF 的规章的效果并且更好在骨胳的肌肉理解它的机制，并且支持 bFGF 的临床的应用治疗骨胳的肌肉疾病，等等相关到肌肉发达的营养障碍或爱滋病， recombinant 人 bFGF ( rh-bFGF )被增加进媒介并且刺激了鼠科的 C2C12 myoblasts 在规章的机制在 myostatin 基因表示和细胞外的调整信号的 kinase 1/2 ( ERK1/2 )的角色的抑制上调查 bFGF 的剂量依赖者效果。同时，完成编码顺序羊似 18 kDa-bFGF 基因被插入到真核细胞的向量 pCMV-neo (从 pEGFP-N1 向量， EGFP 基因从被移开发源) ， recombinant 原生质标志 pCMV-neo-bFGF 被收获并且把以后的手足的骨胳的肌肉注入了老鼠。在鼠科的 C2C12 myoblasts 和骨胳的肌肉的 bFGF， myostatin，和 ERK1/2 基因的表示层次被即时反向的抄写聚合酶链反应和西方的弄污分析分别地分析。结果证明 bFGF 在 C2C12 房间以一种剂量依赖者方式损害了 myostatin 基因的表示，与增加 rh-bFGF 的集中， myostatin mRNA 逐渐地衰退了。另外，在骨胳的肌肉的结果显示 bFGF 也在 vivo 压制了 myostatin 基因的表示。而且，我们发现 ERK1/2 在 myostatin 基因的表示上参予了 bFGF 的规章的机制。

英文摘要：

Basic fibroblast growth factor （bFGF, FGF-2） has an inhibitory effect on the expression of the myostatin gene in murine C2C12 myoblasts, as shown in our recent investigation. To further verify the regulatory effects ofbFGF on the myostatin gene and to better understand its mechanism in skeletal muscle, and to promote clinical applications of bFGF to treat skeletal muscle diseases correlated to muscular dystrophy orAIDS and so on, recombinant human bFGF （rh-bFGF） was added into media and stimulated murine C2C12 myoblasts to investigate the dose-dependent effect of bFGF on suppression of myostatin gene expression and the role of extracellular signal-regulated kinase 1/2 （ERK1/2） in the regulatory mechanism. Simultaneously, complete coding sequence of ovine 18 kDa-bFGF gene was inserted into eukaryotic vector pCMV-neo （originated from pEGFP-N1 vector, from which the EGFP gene has been removed）, the recombinant plasmid pCMV-neo-bFGF was harvested and injected into the mouse skeletal muscle of posterior limb. Expression levels of bFGF, myostatin, and ERK1/2 genes in murine C2C12 myoblasts and the skeletal muscle were analyzed by real-time reverse transcription-polymerase chain reaction and Western blotting analysis, respectively. The results showed that bFGF impaired the expression of myostatin gene in a dose-dependent manner in C2C12 cells, with increasing concentration of rh-bFGF, myostatin mRNA declined gradually. In addition, results in skeletal muscle indicated that bFGF also suppressed the expression of the myostatin gene in vivo. Furthermore, we found ERK1/2 participated in the regulatory mechanism of bFGF on the expression of the myostatin gene.