中文摘要：

【目的】探究Ac SERK1启动子的功能,有助于了解Ac SERK1的表达调控模式。【方法】以‘神湾’菠萝（Ananas comosus L.‘Shenwan’）为材料,将Ac SERK1启动子缺失序列与GUS融合,构建植物表达载体,并导入根瘤农杆菌GV3101中。利用农杆菌真空渗透法侵染烟草叶片,并检测GUS活性;利用浸染法转化菠萝胚性愈伤组织获得转基因植株,分析光照、2,4-D和4℃等处理后的GUS表达量。【结果】构建2个Ac SERK1启动子植物缺失表达载体,分别命名为p（-30/＋258 bp）和p（-499/＋258 bp）。烟草瞬时表达结果显示p（-499/＋258 bp）表现出强的GUS活性,p（-30/＋258 bp）表现出微弱的GUS活性。q RT-PCR结果表明,光照处理后,GUS表达量降低。相反的,2,4-D和4℃处理转基因菠萝植株后GUS表达量均显著增加。【结论】Ac SERK1启动子-499/-30 bp区段内含有光、生长素和低温响应元件。

英文摘要：

【Objective】As a somatic embryogenesis-specific gene, SERK（somatic embryogenesis receptor-like kinase） plays a key role in the development of somatic cells. Analysis of the 5’ upstream region of Ac SERK1 will help us better understand its transcription regulation mechanism. Deletion expression of 5’-flanking region is one of the most significant methods to analyze its promoter function. In this study, deletion expression vectors of the 5’-flanking region fused with a GUS reporter were constructed and then analyzed for the transient expression in tobacco leaves. For further analysis of the promoter function, the stable inherited transgenic pineapple was obtained through using the Agrobacterium-mediated transformation. The putative transgenic plants were analyzed using the molecular biological method. The function of the Ac SERK1 promoter was analyzed by comparing the expression patterns of the reporter gene in transgenic plants which were treated under different conditions.【Methods】The materials used in this experiment were the embryogenic callus of the‘Shenwan’. The Ac SERK1 promoter was truncated at the position of p（-30/＋258 bp） and p（-499/＋258 bp） according to the prediction putative of cis-acting elements and the sites of restriction enzyme which were analyzed by using primer5. These 2 fragments and the p BI121 vector were digested by using Hind Ⅲ and Xba I. After the Ca MV 35 S promoter of p BI121 were replaced by these 2 fragments, the-30/＋258 bp and-499/＋258 bp fragments were fused with the GUS reportergene to construct the plant expression vectors. These recombinant plasmids and p BI121 were transformed into agrobacterium tumefaciens by using the heat-shock method. For the transient expression, the tobacco leaves were infected through Agrobacterium-mediated vacuum infiltration and the GUS activities were examined. After that, the recombinant plasmids were transferred into embryogenic callus by agrobacteriummediated transformation. The kanamycin-resistant regenerated