中文摘要：

目的：探讨折光法在大孔树脂纯化工艺中应用的可行性。方法：选取枳实提取物为研究对象,经AB-8型大孔吸附树脂精制有效部位,用水与50%乙醇洗脱,间隔取样,在线监测洗脱液的折光率并分析其变化规律,同时以HPLC和UV检测作为参比,判断洗脱过程中始点和终点。结果：枳实有效部位精制过程中水洗终点为第4 BV（折光率0）,亦即50%乙醇洗脱始点,50%乙醇洗脱终点为第9 BV（折光率16）。结论：折光法准确可靠、便捷快速,具有较强的实践指导意义。

英文摘要：

Objective：To explore application feasibility of refractometry in purification technology of macroporous resin.Method：Taking extract of Citrus aurantium as research object,active ingredients in it was refined by AB-8 macroporous resin,washed with water and 50% ethanol,interval sampled,refractive ratio of eluent was monitored on line and analyzed its regularity.At the same time,HPLC and UV spectrophotometric method was used to determine hesperidin as references,and to judge initiation point and terminal point during elution.Result：In purification process of active ingredients from C.aurantium,water elution terminal point 4 BV（refractive index 0） of which was the initiation point by 50% ethanol,9 BV（refractive index 16） of 50% ethanol elution was the terminal point.Conclusion：Refractometry was accurate,reliable,convenient,quickly in purification technology of active ingredients of TCM,it had a strong practical significance.