中文摘要：

目的：探讨有丝分裂相关激酶Plk1磷酸化修饰Tara蛋白在调控HeLa细胞胞质分裂及增殖中的作用。方法：运用酵母双杂交、免疫共沉淀、体外沉降实验确定Plk1与Tara两种蛋白是否能够相互结合;采用体内外磷酸化实验明确Plk1对Tara蛋白的磷酸化修饰;采用免疫荧光实验及流式细胞术检测Tara蛋白的磷酸化对HeLa细胞胞质分裂及细胞增殖的影响。结果：Plk1与Tara在体内外均能结合;Tara蛋白在体内外均能够被Plk1磷酸化修饰;过量表达Tara蛋白的磷酸化突变体对HeLa细胞的胞质分裂有明显的抑制作用,细胞增殖明显受阻。结论：Plk1能够与Tara结合,并通过磷酸化修饰Tara蛋白阻碍细胞胞质分裂进程及抑制细胞增殖。

英文摘要：

Aim：To investigate the effects of phosphorylation of Tara by Plk1 on cytokinesis and proliferation of cervical cancer HeLa cells. Methods：Using yeast hybridization assay,co-immunoprecipitation and GST pull-down assay to determine the domain of Plk1 and Tara binding and interaction in vitro and in vivo. To check the phosphorylation of Tara by Plk1 using in vitro phosphorylation assay. Immunoflurescence assay and flow cytometry were used to analyze the effects of phosphorylation of Tara on cytokinesis and proliferation of Hela cells. Results：Plk1 interacted with and phosphorylated Tara in vivo and in vitro. Overexpression of phospho-mimicking mutant of Tara in Hela cells resulted in significant mitosis delay. Conclusion：Plk1-mediated phosphorylation of Tara is essential for cytokinesis and proliferation of HeLa cells.