中文摘要：

决定抄写因素的有约束力的地点为 transcriptional 规定的理解是重要的。抄写因素 c6 月戏在细胞生长，区别和开发的一个重要角色，而是有约束力的地点和目标基因清楚地没在整个人被定义染色体。在这研究，我们执行了一个 ChIP-Seq 实验在人的染色体识别 c6 月绑定地点。48 个有约束力的地点被选择由 dsDNA microarray 试金处理进一步的评估。我们在 K562 房间识别了 283 个 c6 月绑定地点。数据分析证明 48.8% 有约束力的地点在 100 以内定位了 kb 注解基因在上游， 28.6% 有约束力的地点包括了一致 TRE/CRE 主题(5-TGAC/GTCA-3， 5-TGACGTCA-3 ) 并且变体序列。42 从选择了地点被发现在 dsDNA microarray 分析绑 c6 月 homodimer 的 48 绑定。数据分析也证明 1569 基因在 dsDNA microarray 验证的 42 个有约束力的地点的邻居位于 283 个有约束力的地点和 191 基因的邻居。我们在以前的研究请教了 38 c6 月目标基因， 16 也在这研究在这 38 基因之中被检测。在染色体规模的 c6 月绑定地点和潜在的目标基因的鉴定可以在位于与 c6 月有关的抄写规定下面的分子的机制改进我们的基本理解。

英文摘要：

Determining the binding sites of the transcription factor is important for understanding of transcriptional regulation. Transcription factor c-Jun plays an important role in cell growth, differentiation and development, but the binding sites and the target genes are not clearly defined in the whole human genome. In this study, we performed a ChIP-Seq experiment to identify c-Jun binding site in the human genome. Forty-eight binding sites were selected to process further evaluation by dsDNA microarray assay. We identified 283 c-Jun binding sites in K562 cells. Data analysis showed that 48.8% binding sites located within 100 kb of the upstream of the annotated genes, 28.6% binding sites comprised consensus TRE/CRE motif （5′-TGAC/GTCA-3′, 5′-TGACGTCA-3′） and variant sequences. Forty-two out of the selected 48 binding sites were found to bind the c-Jun homodimer in dsDNA microarray analysis. Data analysis also showed that 1569 genes are located in the neighborhood of the 283 binding sites and 191 genes in the neighborhood of the 42 binding sites validated by dsDNA microarray. We consulted 38 c-Jun target genes in previous studies and 16 among these 38 genes were also detected in this study. The identification of c-Jun binding sites and potential target genes in the genome scale may improve our fundamental understanding in the molecular mechanisms underlying the transcription regulation related to c-Jun.