中文摘要：

建立了生物质谱研究rhEPO 一级结构的系列方法,包括分子量、唾液酸含量以及N-糖含量等糖基化性质的测定方法,并用于4种国产rhEPO 样品的分析;在此基础上优化酶切条件,使测定的肽质量指纹谱（PMF）的覆盖率均达到98%,成功鉴定了4个样品中的全部N-糖基化位点以及对分子中两对二硫键进行了结构确证,测定4个样品分子量分别为：27754.1±27.4Da、27941.4±23.2 Da、27682.5±22.0 Da和27914.7±22.5 Da;唾液酸含量分别为：5.0%、4.8%、5.6%和5.4%;糖含量分别为：34.3%、34.7%、34.1%和34.6%.此外还对4种产品在O-连接糖型及N-连接糖型上的区别做了初步探讨.

英文摘要：

A series of method for the primary structure determination of recombinant human erythropoietin （rhEPO）, a recombinant glycoprotein, by matrix-assisted laser desorption ionization-time of flight-time of flight-mass spectrometry （MALDI-TOF-TOF-MS） was developed , which includes the methods of the measurement of molecular weight, content of sialic acid and carbohydrate in rhEPO etc. Based on the developed methods, four kinds of rhEPO coming from different manufactories were determined. The coverage of PMF of all rhEPOs reached 98% by optimization of digestion condition, and N-glycan site and two disulphide bridges in the rhEPOs were determined successfully. Molecular weight of four samples are （27754. 1 ± 27.4） Da, （27941.4 ±23.2）Da, （27682.5 ±22.0） Da and （27914.7 ±22.5） Da; content of sialic acid are 5.0%, 4. 8%, 5.6% and 5.4% ; and content of carbohydrate are 34.3%, 34.7% , 34.1% and 34.6%. Moreover, the difference of the glycan structure type on among these four rhEPOs was probed and discussed.