位置:成果数据库 > 期刊 > 期刊详情页
香蕉条斑病毒CP区基因的克隆及其在大肠杆菌中的融合表达
  • 期刊名称:安徽农业科学,2007,35(23):7108-7109
  • 时间:0
  • 分类:Q781[生物学—分子生物学] S435.111.4[农业科学—农业昆虫与害虫防治;农业科学—植物保护]
  • 作者机构:[1]Agricultural Ministry Key Laboratory of Tropical Crop Biotechnology, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China, [2]Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China, [3]Environment and plant protection institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571734, China
  • 相关基金:Foundation items: Nature Science Foundation of China (No. 30660100); National Key Technology R&D Program of China (2007BAD48B01); and National Nonprofit Institute Research Grant of CATAS-ITBB (ITBB110304).
  • 相关项目:香蕉条斑病毒侵染性克隆及基因组结构与功能研究
中文摘要:

香蕉条纹病毒(BSV ) ,类 Badnavirus 的一个成员,是在全世界的香蕉条纹疾病的一个原因的代理人。从香蕉种植园的不同区域的 BSV 的基因差异以前被调查了,但是有相对 episomal 的基因特征的很少报告(非综合) 从中国孤立的 BSV 染色体。这里,完全的染色体, 7722bp (GenBank 就职数字 DQ092436 ) 的一个总数,一在云南的栽培变种卡文迪什(BSAcYNV ) 上的香蕉条纹病毒(BSV ) 的 isolate,中国被决定。染色体以 badnaviruses 的典型方式组织。genomic DNA 的 intergenic 区域包含一个大茎环,它可以贡献核糖体移动进下列开的读物框架(ORF ) 。BSAcYNV 的编码区域由三重叠 ORF 组成,有编码二小蛋白质的非 8 月开始 codon 和 ORF2 的 ORF1 个别地涉及病毒的运动, ORF3 编码 polyprotein。除完全的染色体以外,缺乏整个 RNA 领导人区域和 ORF1 的一个多数的一个有缺点的染色体并且它包含 6525bp 也在感染的香蕉植物从这座 BSV DNA 水库被孤立并且定序。顺序分析证明 BSAcYNV 与 BSV 以染色体组织和编码任务有最靠近的类似从越南(BSAcVNV ) 孤立。相应编码区域分别地在核苷酸和氨基酸层次分享了 88% 和 95% 的身份。种系发生的分析也显示 BSAcYNV 在定序的香蕉条纹 badnaviruses 之中分享了最近地理的进化关系到 BSAcVNV。

英文摘要:

Banana streak virus (BSV), a member of genus Badnavirus, is a causal agent of banana streak disease throughout the world. The genetic diversity of BSVs from different regions of banana plantations has previously been investigated, but there are relatively few reports of the genetic characteristic of episomal (non-integrated) BSV genomes isolated from China. Here, the complete genome, a total of 7722bp (GenBank accession number DQ092436), of an isolate of Banana streak virus (BSV) on cultivar Cavendish (BSAcYNV) in Yunnan, China was determined. The genome organises in the typical manner of badnaviruses. The intergenic region of genomic DNA contains a large stem-loop, which may contribute to the ribosome shift into the following open reading frames (ORFs). The coding region of BSAcYNV consists of three overlapping ORFs, ORF 1 with a non-AUG start codon and ORF2 encoding two small proteins are individually involved in viral movement and ORF3 encodes a polyprotein. Besides the complete genome, a defective genome lacking the whole RNA leader region and a majority of ORF1 and which encompasses 6525bp was also isolated and sequenced from this BSV DNA reservoir in infected banana plants. Sequence analyses showed that BSAcYNV has closest similarity in terms of genome organization and the coding assignments with an BSV isolate from Vietnam (BSAcVNV). The corresponding coding regions shared identities of 88% and -95% at nucleotide and amino acid levels, respectively. Phylogenetic analysis also indicated BSAcYNV shared the closest geographical evolutionary relationship to BSAcVNV among sequenced banana streak badnaviruses.

同期刊论文项目
同项目期刊论文