中文摘要：

目的：探讨RECK蛋白、膜型基质金属蛋白酶-1（MT1-MMP）在肝母细胞瘤组织中的表达及其在肿瘤转移抑制中的作用。方法：采用第二代通用型二步法监测系统（PV-6000）免疫组织化学方法检测35例肝母细胞瘤组织和15例正常肝脏组织及10例肝脏良性肿瘤组织中RECK、MT1-MMP的表达情况。结果：肝母细胞瘤组织RECK的表达水平明显降低（28.6%）;MT1-MMP肝母细胞瘤组织中高表达（54.3%）,并随着肿瘤浸润深度的加深、远处转移的发生而增高（p〈0.05）;二者表达呈负相关（p〈0.05）。结论：RECK和MT1-MMP在肝母细胞瘤组织中的表达呈负相关;RECK和MT1-MMP在肝母细胞瘤的进展中起重要作用。

英文摘要：

Objective：To investigate the expression of kazal motifs（RECK） and membrane-type1 matrix metalloproteinase（MT1-MMP） in hepatoblastoma and their correlation with metastasis and suppression in the tumor. Methods：PV-6000 immunohistochemistry method was used to detect the expression of RECK and MT1-MMP in 35 samples of hepatoblastoma tissue,15 cases of normal liver tissues and 10 cases of benign liver tumor tissues. Results：The expression of the RECK protein in the hepatoblastoma decreased significantly（28.6%） . The expression of the MT1-MMP in the hepatoblastoma was high（54.3%） and increased with the increase in the extent of invasive depth and metastasis（P0.05） . There was a negative correlation between the expression of the RECK and MT1-MMP in hepatoblastoma（ P 0.05） . Conclusions：RECK and MT1-MMP may play important roles in the occurrence,development,invasion and metastasis of hepatoblastoma and serve as markers in the estimation of the extent of metastasis and dissemination of the hepatoblastoma.