中文摘要：

目的探讨大麻素受体2（cannabinoid receptor2，CB2R）激动剂JWH015对瑞芬太尼诱发的切口痛模型大鼠痛觉过敏的影响。方法60只雄性sD大鼠采用随机数字表法随机分成2大组：鞘内给药组和腹腔给药组。每大组又随机分为5小组：鞘内给药组分为对照组1（C1组）、切口痛组1（I1组）、瑞芬太尼组1（R1组）、切口痛＋JWH015组（QI组）、切口痛＋瑞芬太尼＋JWH015组（QR组）；腹腔给药组分为对照组2（C2组）、切口痛组2（12组）、瑞芬太尼组2（R2组）、切口痛＋JWH015组（FI组），切口痛＋瑞芬太尼、＋JWH015组（FR组），每组6只。QI组与QR组在造模前30min鞘内注射10ug JWH015，C1、I1、R1组均鞘内给予20％的二甲基亚砜（DMSO）溶液，容积均为10ul；而FI组与FR组在造模前30min腹腔注射100ug JWH015，C2、12、R2组均腹腔给予4％的DMSO溶液，容积均为0．5ml。除C1／C2组外，其余各组均制作切口痛模型，R1／R2组、QR组和FR组在造模的同时皮下泵注瑞芬太尼0．04mg／kg，其余组皮下泵注生理盐水，容积均为0．4ml，30min泵完。测量术前24h及术后2，6，24，48h大鼠手术切口同侧后爪的机械缩足反射阈值（PWMT）及热缩足潜伏期（PWTL）。结果与C1／C2组和基础值比较，11／12组术后PWMT，和PWTL均降低（P〈0．01）；与11／12组比较，R1／R2组术后PWMT和PWTL均明显降低（P〈0．05）；与R1／IR2组相比，QR组从术后6h的PWMT[（7．78±1．09）g]和PWTL[（17．28±1．58）S]开始明显升高（P〈0．05），与FR组在术后6h、24h和48h的PWMT[（7．79±0．72）g，（9．50±1．17）g，（7．86±1．16）g]和PWTL[（16．23±1．50）s，（19．53±1．63）S，（18．10±0．93）s]升高的结果一致。结论鞘内及腹腔注射JWH015均可以有效缓解由瑞芬太尼诱发的切口周围组织痛觉过敏。

英文摘要：

Objective To investigate the effects of cannabinoid receptor 2 （cannabinoid receptor 2, CB2R） agonist JWHO15 on the hyperalgesia induced by remifentanil in a rat model of postoperative pain. Methods Sixty SD rats were randomly divided into 10 groups （ n = 6 each） ：control groups （C1 and C2）,incisional pain groups （I1 and I2） , incisional pain plus JWHO15 groups （QI and FI）, remifentanil groups （R1 and R2）, and JWHO15 plus remifentanil groups （ QR and FR）. Rats in group QI/QR and FI/FR were intrathccal injection with lOp.g JWHO15 （ diluted in 10ul 20% DMSO solution） and intraperitoneal administration with 100ug JWHO15 （ diluted in 10ul 4% DMSO solution） respectively 30 min before plantar incision while rats in group C ,I and R were received with the same volume of DMSO solution. Plantar incision surgery was operated in rats of group I, R, QI/ FI,and QR/FR. In group R and QR/FR,remifentanil （0.04 mg/kg） was infused subcutaneously to rats with a pump for 30 min at the moment of surgical incision. The paw withdrawal mechanical threshold （PWMT） and paw withdrawal thermal latency （PWTL） at 24 h before incision and at 2 h,6 h,24 h and 48 h after incision were tested to evaluate the behavioral changes. Results Compared with group C and baseline, the level of PWMT and PWTL decreased at 2 h,6 h,24 h and 48h after incision in group I （P〈0.01） ;Compared with group 1 ,the significant decrease of PWMT and PWTL were observed after incision in group R （P 〈 0.05 ） ; Compared with group R, the significant increase of PWMT （ 7.78 ± 1.09 ） and PWTL （ 17.28 ± 1.58 ） were observed from 6 h after incision in group QR（P〈 0.05）. And the increase of PWMT （7.79 ±0.72,9.50 ± 1. 17,7.86 ± 1. 16） and PWTL （ 16.23 ± 1.50,19.53 ± 1.63,18.10 ± 0.93） were observed at 6 h,24 h and 48 h after incision in group FR（P〈 0.05 ）. Conclusion Intrathecal and intraperitoneal administration of JWH015 in this investigation dose could relief remifentanil-induced