中文摘要：

目的：了解脓毒症时肠道谷氨酰胺（Gln）转运的变化,研究小檗碱对脓毒症时肠道Gln的调节作用。方法：采用盲肠结扎穿孔法建立大鼠脓毒症模型,将18只大鼠随机分为对照组、模型组和治疗组。小檗碱用量50mg/kg,每天灌胃1次,第5天造模。造模后0、2、6、12和24h取材,用Mg2＋沉淀/差速离心法制备刷状缘膜囊。采用快速滤过技术检测Gln转运,并利用底物竞争性抑制法测定各转运体的转运活性。结果：在生理状态下,Na＋依赖性Gln转运约占总转运量的90%,其中ATA2、ATB0,＋和B0AT1分别占12%、25%和63%。脓毒症早期肠道Gln转运增加,后期下降,其中ATA2的升高最早出现,B0AT1早期无显著升高。小檗碱处理后肠道Gln转运先升高后下降的趋势明显减弱。结论：脓毒症时肠道Gln转运早期增加后期减少,小檗碱处理后可减轻脓毒症对肠道Gln转运的损伤。

英文摘要：

Objective：A marked deficiency of glutamine in clinical critical illness is correlated with mortality in the intensive care unit, and intestinal glutamine transport was reported to be impaired in late sepsis. Berberine was reported to protect against the intestine injury, and improve the survival rate in sepsis. We designed this study to gain further knowledge of the intestinal glutamine transport in early and late sepsis, and to find out whether beberine pretreatment has some effect on glutamine transport in sepsis. Methods： Berberine （50 mg/kg） was given intragastrically once a day for 5 days, and sepsis was induced by cecal ligation and puncture on day 5. The small intestinal samples were collected at 0, 2, 6, 12, 24 h. Intestinal brush border membrane vesicles were prepared by Mg2＋ aggregation-differential centrifugation techniques, and brush border glutamine transport was studied by a rapid filtration technique. Results： Under control condition, Na＋-dependent glutamine transport accounted for about 90% of the total transport. The relative contributions of ATA2, ATB0,＋, B0AT1 were about 12, 25, 63%, respectively. Septic rats showed an early increase and a late decrease in intestinal glutamine transport. ATA2 had an earlier increase in the early stage, while B0AT1 had no significant increase. Berberine pretreated group had a relative less increase in early phase and a less decrease in late phase compared to sepsis group. Conclusion： Rat intestinal glutamine transport showed an early increase and a late decrease in sepsis, and berberine pretreatment could attenuate the impairment of glutamine transport in sepsis. It may provide some information for sepsis treatment.