中文摘要：

在克隆水稻编码磷脂酰丝氨酸合酶的短穗颈基因SUI1的基础上,利用Gateway方法分别构建了N端和C端两种GFP融合载体pMDC45-SOVT和pMDC201-SOV。利用农杆菌侵染烟草叶片的方法,观察SUI1蛋白在烟草叶肉细胞中的瞬时表达情况,结果显示GFP-SUI1定位于质膜和核上,而SUI1-GFP定位于质膜和核膜上,并且两种融合蛋白在质膜上的分布都是不连续的。由于N端融合可能改变了目标蛋白的折叠方式及跨膜方式,从而影响蛋白的正确定位,因而SUI1蛋白主要定位于烟草叶肉细胞的质膜和核膜中。

英文摘要：

SUI1 encodes a phosphatidylserine synthase.And its mutant sui1-1 exhibites a shortened uppermost internode and apartly sheathed panicle.On this basis,two vectors pMDC45-SOVT and pMDC201-SOV were constructed by the Gateway system,which responds to the N-terminal and C-terminal fusion GFP.To observe the subcellular localization of SUI1,tobacco leaves have been infected by A grobacterium with destination vectors.The results showed that GFP-SUI1 fusion proteins positioned in the plasma membrane and nucleus,while SUI1-GFP were localized in the plasma membrane and the nuclear membrane.Moreover,the distribution of the two fusion proteins were not continuous in the plasma membrane.We speculate that SUI1 protein was localized in the plasma membrane and nuclear membrane of tobacco leaf cells because the target protein folding and transmembrane manner may be changed by the N-terminal fusion,affecting the correct position of the protein.