中文摘要：

鞘脂激活蛋白原（prosaposin）是一种多功能的糖蛋白。除作为鞘脂糖水解途径中所涉及到的溶酶体酶的激活剂外，其在神经系统、生殖系统及前列腺癌的发生中都担当重要角色。利用酵母双杂交系统筛选与Rhox5蛋白相作用的分子，获得一个阳性克隆No．1—14，序列比对表明其是prosaposin（Psap）的C末端。进一步的研究表明，全长的prosaposin（不含外显子8的剪接本）亦可以与Rhox5相互作用。暗示prosaposin（Psap）的C末端可能是Psap与Rhox5相互作用的关键结构。由于Psap与Rhox5均在生殖系统的发生、发展和分化，精子的生成和成熟中发挥重要作用，Psap与Rhox5相互作用的证实提示两者可能共同参与雄性生殖系统发生的动态调控。

英文摘要：

Prosaposin （Psap） has multiple cellular functions. It is involved in the development of the reproductive system, nervous system, and prostate cancer as well as in the regulation of sphingolipid catabolism by activating several lysosomal hydrolases involved in the metabolism of various sphingolipids. In this research, it was found to be a novel interaction partner for Rhox5 using yeast two-hybrid screening. The interaction between Rhox5 and the full-length prosapsoin （the transcript without exon 8） as well as the C-terminal domain of prosaposin, was further confirmed in both yeast two hybrid analysis and in vitro assay. It suggested that the C-terminal domain of prosaposin may be critical for the Rhox5-prosaposin interaction. Given the important roles played by both Rhox5 and prosaposin in maintaining the differentiation of male reproductive organs, spermatogenesis, and fertilization, the interaction between Rhox5 and prosaposin might regulate the development of male reproductive organs dynamically.