中文摘要：

应用已知的标签序列通过RACE-PCR和RT-PCR方法成功克隆红笛鲷（Lutjanus sanguineus）α-珠蛋白和β-珠蛋白基因的全长cDNA序列。α-珠蛋白和β-珠蛋白基因的cDNA全长分别为560和563 bp,开放阅读框分别为429和444 bp,各编码143和148个氨基酸,理论相对分子质量分别为15690和16400,理论等电点为7.79和6.61。氨基酸序列BLAST结果表明,红笛鲷α-珠蛋白基因和β-珠蛋白基因编码的氨基酸序列与其他鱼类的相似性分别在59%-79%和55%-80%之间。用邻接法（Neighbor-Joining）构建的α-珠蛋白基因和β-珠蛋白基因的系统发育树表明,红笛鲷与真鲷（Pagrus major）、金头鲷（Sparus aurata）和鰤（Seriola quinqueradiata）等亲缘关系较近。

英文摘要：

Full-length cDNA sequence of α-globin and β-globin gene of humphead snapper（Lutjanus sanguineus） were cloned by RACE-PCR and RT-PCR method from the known ESTs.The length of α-globin and β-globin gene are 560 bp and 563 bp respectively.The open reading frame of α-globin and β-globin gene contain 429 bp and 444 bp respectively,and encode 143 and 148 amino acids respectively.Relative molecular weight of α-globin and β-globin are 15 690 and 16 400.The theoretical isoelectric point of them are 7.79 and 6.61,respectively.Results of amino acid sequence analysis show that the similarity of α-globin and β-globin gene of humphead snapper compared with other fish are in the range of 59 %-79 % and 55 %-80 % respectively.Phylogenetic tree of α-globin and β-globin gene are built by neighbor-joining method and the results also show that humphead snapper has the closest homology with Pagrus major,Sparus aurata and Seriola quinqueradiata.