采用田间试验与室内分析相结合的方法,研究了氮肥形态对小麦不同生育期土壤酶活性的影响。结果表明:脲酶、蛋白酶活性随小麦生育时期推进呈现出两个峰值,蔗糖酶先降后升再降,呈倒‘S’型曲线,脱氢酶在越冬、成熟期高,其它生育期低,曲线似‘U’型,过氧化氢酶活性在返青后呈急剧上升,拔节后缓降。0~20 cm酶活性普遍高于20~40 cm;施肥可以提高土壤酶活性,可使脲酶活性提高60.3%,蛋白酶活性平均提高36.0%,蔗糖酶活性平均提高36.7%,脱氢酶活性平均提高100%,过氧化氢酶平均提高33.8%;土壤酶活性变异系数的平均值大小顺序是:脲酶>脱氢酶>蛋白酶>蔗糖酶>过氧化氢酶;氮肥形态对小麦不同生育期5种土壤酶的影响各异,没有一定的规律性。
A field experiment was carried out to study the effects of nitrogen (N)fertilizer forms on soil enzyme ac-tivities at different growth stages of wheat .The results showed,during the whole growing season of wheat,the activities of urease and protease presented a two-peak curve,while the activities of invertase,dehydrogenase and catalase demon-strated an inverted S-curve (i .e .,decreasing-rising-decreasing),U-curve (i .e .,being high at overwintering and maturity stage but low at other stages),and inverted L-curve (i .e .,being risen sharply after reviving stage but de-creased slowly after jointing stage),respectively .The enzyme activities in 0~20 cm layer of soil were generally higher than those in 20~40 cm layer .N application significantly improved soil enzyme activities,in which the activities of ure-ase,protease,invertase,dehydrogenase and catalase were elevated by 60 .3%,36 .0%,36 .7%,100%and 33 .8%on average,respectively .The average values of coefficient of variation of soil enzyme activities were sequenced as:urease〉dehydrogenase 〉protease 〉invertase 〉catalase .The effects of N fertilizer forms to soil enzyme activities at differ-ent stages of wheat were different,but there was no definite regularity .