中文摘要：

利用降落PCR的方法,从矮牵牛DNA中扩增出ACS2基因片段,在GenBank中获得基因序列号为DQ090003 利用一步法将ACS2基因的反义重复与pBI-121质粒连接,构建含该基因的反义重复表达载体。

英文摘要：

Used the method of touch down PCR, the ACS2 gene was cloned from DNA of Petunia hybrida. At the same time, its gene sequence DQ090003 was obtained in GenBank, the antisense repetition of ACS2 linked the plasmid pBI-121 by use of one step. In the result, the expression vector having antisense repetition gene was constucted.