中文摘要：

目的探讨乙肝病毒X基因（HBx）对人肝癌细胞原癌基因ras表达水平的影响。方法将pcDNA3.1载体与HBx基因连接构建真核表达载体pcDNA3.1-HBx,采用脂质体介导转染人肝癌细胞Bel7402,以转染空载体pcDNA3.1的Bel7402细胞为对照,于转染后2、4、8、16 h收集细胞,提取细胞总蛋白并用Western blot技术检测肝癌细胞内n-Ras蛋白表达水平。结果与转染空载体pcDNA3.1的Bel7402对照组相比,转染重组表达载体pcDNA3.1-HBx的Bel7402细胞Ras蛋白表达水平在第2小时明显降低,第4小时Ras蛋白的表达水平仍有降低趋势,但比第2小时表达量有所增加;第8小时和第16小时,Ras蛋白的表达水平与对照组相比明显升高,差异有统计学意义（P〈0.05）。结论 HBx对人肝癌Bel7402细胞Ras蛋白表达有明显影响,HBx持续表达可能对Ras表达有促进作用。

英文摘要：

Objective To investigate the influence of HBx on proto-oncogene ras expression in human hepatoma cells.Methods HBx linked to pc DNA3.1 for constructing eukaryotic expression vector pc DNA3.1-HBx. pc DNA3.1-HBx vectors were transfected into Bel7402 cells to establish Bel7402/HBx cell model. Bel7402 cells transfected with pc DNA3.1 was used as control. Western blot was applied to evaluate the expression levels of n-Ras in Bel7402 cell while transfected with pc DNA3.1-HBx vectors for 2, 4, 8, 16 h. Results The expression of Ras in Bel7402 was significantly lower while transfected with pc DNA3.1-HBx vectors for 2 h and 4 h contrast with control group（vehicle vector）, however, the expression of Ras was significantly promoted while transfected with pc DNA3.1-HBx vectors for 8 h and 16 h（P〈0.05）. Conclusion HBx plays a role in influencing the expression of Ras in Bel7402 cells. Persistent expression of HBx maybe stimulate expression of Ras in hepatoma cells.