中文摘要：

毛囊生长周期中,真皮乳头和毛基质间的基质-上皮信号调控细胞的增殖和分化。多功能细胞调控因子胰岛素样生长因子1（IGF1）是该信号路径的成员之一。第1个毛囊生长周期决定着毛囊的正常生长和发育,但IGF1在此期的作用未见报道。实时荧光定量PCR结果显示,IGF1在生长期皮肤中的相对表达量最低,在退化期表达量最高,在静止期表达量又降低。与生长初期相比,IGF1在退化期和静止期的表达量呈差异极显著（P〈0.01）;胰岛素样生长因子1受体（IGF1R）在生长期皮肤中的相对表达量最高,在退化期表达量最低,而在静止期表达量又升高。与生长初期相比,IGF1R在退化期和静止期的表达量呈差异极显著（P〈0.01）。Western印迹结果显示,IGF1和IGF1R蛋白在小鼠皮肤第1个毛囊生长周期各阶段的表达趋势分别与其mRNA的表达趋势一致;免疫组织化学结果表明,IGF1主要分布在小鼠表皮,而IGF1R免疫阳性在小鼠毛囊毛球部、内外根鞘和毛乳头均有分布。以上实验结果揭示,IGF1和IGF1R在小鼠皮肤第1个毛囊生长周期的各阶段的差异性表达,可能在毛囊生长周期各阶段的转化过程中参与了黑色素的形成。然而,IGF1和IGF1R表达趋势不一致,提示IGF1在小鼠皮肤中发挥作用时,并非只与IGF1R结合才能发挥作用。

英文摘要：

Mesenchymal-epithelial signaling between the dermal papilla and the hair matrix regulates cell proliferation and differentiation during the hair follicle cycle. Insulin-like growth factor 1（ IGF1） is one of the components of this signaling pathway; it is a multifunctional regulator molecule in cells. The first hair follicle cycle is the most important determinant for the normal growth and development of hair follicles,but the function of IGF1 has not been reported. The expression of IGF1 and the IGF1 receptor（ IGF1R）in the skins undergoing the first hair follicle cycle was measured to investigate the function of IGF1 and IGF1 R in mice. Quantitative real time PCR analysis showed that the mRNA expression level of IGF1 was at its lowest at anagen,highest at catagen,and decreased from telogen（ but remained higher than that of early anagen）. In comparison to the mRNA expression level of IGF1 at early anagen,the expression levels at catagen and telogen was significantly different（ P 0. 01）. The mRNA expression of IGF1 R was highest at catagen,lowest at anagen,and increased at telogen（ but remained lower than that of earlyanagen）. In comparison to the mRNA expression levels of IGF1 R at early anagen,the expression levels at catagen and telogen were significantly different（ P 0. 01）. Western blotting analysis showed that the immune reactive bands corresponding to IGF1 and IGF1 R were detected,and the expression of IGF1 and IGF1 R shared the same trends as the expression of their corresponding mRNA transcripts.Immunohistochemistry analysis showed that IGF1 was expressed mainly in the epidermis,while IGF1 R was localized in the hair dermal papilla,the lower section of the hair bulb,and at the outer and inner root sheath of hair follicles. The expression and localization of IGF1 and IGF1 R at the different stages of the first hair follicle cycle were significantly different,which suggested a potential role of IGF1 in regulating the transformations from anagen through to catagen and telogen may be in